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Titolo:
Molecular characterization of Dau c 1, the Bet v 1 homologous protein fromcarrot and its cross-reactivity with Bet v 1 and Api g 1
Autore:
Hoffmann-Sommergruber, K; ORiordain, G; Ahorn, H; Ebner, C; da Camara Machado, ML; Puhringer, H; Scheiner, O; Breiteneder, H;
Indirizzi:
Univ Vienna, Dept Gen & Expt Pathol, A-1010 Vienna, Austria Univ Vienna Vienna Austria A-1010 & Expt Pathol, A-1010 Vienna, Austria Ernst Bohringer Inst, Vienna, Austria Ernst Bohringer Inst Vienna Austria nst Bohringer Inst, Vienna, Austria Univ Agr Sci, Inst Appl Microbiol, Vienna, Austria Univ Agr Sci Vienna Austria r Sci, Inst Appl Microbiol, Vienna, Austria
Titolo Testata:
CLINICAL AND EXPERIMENTAL ALLERGY
fascicolo: 6, volume: 29, anno: 1999,
pagine: 840 - 847
SICI:
0954-7894(199906)29:6<840:MCODC1>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
BIRCH POLLEN ALLERGEN; PATHOGENESIS-RELATED PROTEINS; MUGWORT-SPICE SYNDROME; HIGH-LEVEL EXPRESSION; MAJOR ALLERGEN; TREE POLLEN; FOOD ALLERGY; ESCHERICHIA-COLI; GRASS-POLLEN; CLONING;
Keywords:
Dau c 1; Bet v 1; Api g 1; food allergen; carrot; IgE-binding; recombinant allergen;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Hoffmann-Sommergruber, K AKH,a,BO, Dept Gen & Expt Pathol, 3Q,Waehringer Guertel 18-20, A-1090 Vienn AKH 3Q,Waehringer Guertel 18-20 Vienna Austria A-1090
Citazione:
K. Hoffmann-Sommergruber et al., "Molecular characterization of Dau c 1, the Bet v 1 homologous protein fromcarrot and its cross-reactivity with Bet v 1 and Api g 1", CLIN EXP AL, 29(6), 1999, pp. 840-847

Abstract

Background Up to 70% of patients with birch pollen allergy exhibit the so-called oral allergy syndrome, an IgE-mediated food allergy. The most frequent and therefore best characterized pollen-fruit syndrome is apple allergy in patients suffering from tree pollen-induced pollinosis. The occurrence of adverse reactions to proteins present in vegetables such as celery and carrots in patients suffering from pollen allergy has also been reported. cDNAs for Bet v I homologous proteins have been cloned from celery, apple and cherry. Objective The aim of the study was to identify Bet v 1 homologues from carrot (Daucus carota), to isolate the respective cDNA, to compare the IgrE-binding capacity of the natural protein to the recombinant allergen and determine the cross-reactivity to Api g 1 and Bet v 1,Methods Molecular characterization of the carrot allergen was performed using IgE-immunoblotting, cross-inhibition assays, N-terminal sequencing, PCR-based cDNA cloning and expression of the recombinant protein in Escherichia coli. Results A 16-kDa protein from carrot was identified as a major IgE-bindingcomponent and designated Dau c 1. Sequencing corresponding cDNAs revealed three extremely similar sequences (Dau c 1.1, 1.2 and 1.3) with an open reading frame of 462 bp coding for 154 amino acid residues. Conclusions Purified recombinant Dau c 1.2 was tested in immunoblots displaying IgE-binding capacity comparable to its natural counterpart. Cross-inhibition assays verified the existence of common B-cell epitopes present on Dau c 1, Api g 1 as well as on Bet v 1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/07/18 alle ore 13:32:05