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Titolo:
Semen specific gamma-glutamyltransferase carries ABH antigens: a sandwich ELISA for simultaneous semen detection and its ABO blood typing
Autore:
Abe, S; Gunji, H; Kunii, S; Kuraya, M; Fujita, T; Hiraiwa, K;
Indirizzi:
Fukushima Med Univ, Dept Legal Med, Sch Med, Fukushima 9601295, Japan Fukushima Med Univ Fukushima Japan 9601295 Med, Fukushima 9601295, Japan Fukushima Med Univ, Dept Biochem, Sch Med, Fukushima 9601295, Japan Fukushima Med Univ Fukushima Japan 9601295 Med, Fukushima 9601295, Japan
Titolo Testata:
CLINICA CHIMICA ACTA
fascicolo: 1-2, volume: 283, anno: 1999,
pagine: 183 - 194
SICI:
0009-8981(199905)283:1-2<183:SSGCAA>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
TISSUE-SPECIFIC EPITOPES; GLUTAMYL-TRANSPEPTIDASE; MONOCLONAL-ANTIBODIES; TRANSFERASE;
Keywords:
semen specific gamma-GTP; ABH antigen carrier protein; monoclonal antibodies; sandwich ELISA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Abe, S Fukushima95,d Univ, Dept Legal Med, Sch Med, Hikarigaoka 1, Fukushima 96012 Fukushima Med Univ Hikarigaoka 1 Fukushima Japan 9601295 ima 96012
Citazione:
S. Abe et al., "Semen specific gamma-glutamyltransferase carries ABH antigens: a sandwich ELISA for simultaneous semen detection and its ABO blood typing", CLIN CHIM A, 283(1-2), 1999, pp. 183-194

Abstract

Semen type of gamma-glutamyltransferase (gamma-GTP) is different from the membrane bound type of the enzyme in both biochemical and immunological properties, and consists of two subunits (150 and 95 kDa). We found that anti-ABH antibodies recognize a 150-kDa subunit of seminal gamma-GTP by Western blot and immunoprecipitation analyses. Using SG2, one of anti-semen specific gamma-GTP monoclonal antibodies which we had produced, and anti-ABH antibodies, we established a sandwich ELISA for identifying human seminal gamma-GTP and its ABO type simultaneously. This sandwich ELISA allows ABO typing of highly diluted semen. The dilutions for ABO typing were 10(5) times for A or O, and 10(4) times for B. Furthermore, ABO typing of semen was successfully performed by this ELISA, even in the mixed presence of vaginal fluid,saliva and blood. Thus, seminal gamma-GTP carries ABH antigens and the sandwich ELISA with SG2 and anti-ABH antibodies enables ABO typing of semen. The sandwich ELISA is extremely useful for ABO typing originated from semen in the mixture of biological fluids. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 09:27:01