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Titolo:
cDNA representational difference analysis of differentially expressed cDNAsequences in human nasopharyngeal carcinoma
Autore:
Zhan, FG; Cao, L; Bin, LH; Jiang, N; Deng, LW; Xie, Y; Tan, GL; Li, GY;
Indirizzi:
Hunan Med Coll, Inst Canc Res, Changsha 410078, Peoples R China Hunan Med Coll Changsha Peoples R China 410078 a 410078, Peoples R China Hunan Med Coll, Affiliated Hosp 3, Changsha 410078, Peoples R China Hunan Med Coll Changsha Peoples R China 410078 a 410078, Peoples R China
Titolo Testata:
CHINESE MEDICAL JOURNAL
fascicolo: 6, volume: 112, anno: 1999,
pagine: 538 - 542
SICI:
0366-6999(199906)112:6<538:CRDAOD>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Keywords:
nasopharyngeal carcinoma; cDNA representational difference analysis; tumor suppressor gene cloning;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Zhan, FG Hunan Med Coll, Inst Canc Res, Changsha 410078, Peoples R China Hunan Med Coll Changsha Peoples R China 410078 Peoples R China
Citazione:
F.G. Zhan et al., "cDNA representational difference analysis of differentially expressed cDNAsequences in human nasopharyngeal carcinoma", CHIN MED J, 112(6), 1999, pp. 538-542

Abstract

Objective To search differentially expressed sequences correlated with pathogenesis of human nasopharyngeal carcinoma (NPC), including the candidatesof tumor suppressor genes. Methods Representational difference analysis (RDA) was performed to isolate differentially expressed sequences between cDNA from normal human primarycultures of nasopharyngeal epithelial cells and cDNA from NPC cell line HNE1. The source of differentially expressed products were proved by Southernblot, Northern blot and in situ hybridization. The fragments were cloned with pGEM-T easy kit and sequenced by the chain termination reaction. Results Four differentially expressed cDNA fragments were isolated in the fourth subtractive hybridization using cDNA from normal human nasopharyngeal epithelial cells as tester amplicon and cDNA from NPC cell line HNE1 as driver amplicon by cDNA RDA. These differential cDNA fragments revealed thatthey really came from the tester amplicon and were not expressed or down-regulated in the NPC HNE1 cells. Some of the genes were expressed only in human nasopharyngeal epithelial cells but deleted or downregulated in the biopsies of NPC. Of these obtained clones, some were the sequences of the human known genes including house-keeping genes, the others represented novel gene sequences. Conclusion The differentially expressed products including the candidates of tumor-suppressor genes may be associated with the initiation of the NPC.

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Documento generato il 08/04/20 alle ore 09:12:58