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Titolo:
Patch clamp recording of the responses to three bitter stimuli in mouse taste cells
Autore:
Seto, E; Hayashi, Y; Mori, T;
Indirizzi:
Kyoto Univ, Food Sci Res Inst, Kyoto 6110011, Japan Kyoto Univ Kyoto Japan 6110011 , Food Sci Res Inst, Kyoto 6110011, Japan
Titolo Testata:
CELLULAR AND MOLECULAR BIOLOGY
fascicolo: 3, volume: 45, anno: 1999,
pagine: 317 - 325
SICI:
0145-5680(199905)45:3<317:PCROTR>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
G-PROTEIN; PHOSPHOLIPASE-C; RECEPTOR-CELLS; TRANSDUCTION; CONDUCTANCE; MODULATION; GUSTDUCIN; QUININE; SWEET; PHOSPHODIESTERASE;
Keywords:
taste; bitter; patch clamp; mouse; quinine; denatonium; naringin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Mori, T Kyoto Univ, Food Sci Res Inst, Kyoto 6110011, Japan Kyoto Univ Kyoto Japan 6110011 ci Res Inst, Kyoto 6110011, Japan
Citazione:
E. Seto et al., "Patch clamp recording of the responses to three bitter stimuli in mouse taste cells", CELL MOL B, 45(3), 1999, pp. 317-325

Abstract

Although several pathways of bitter taste signal transduction have been proposed in taste cells, these mechanisms have not been elucidated in detail,To investigate the diversity of responses to bitter stimuli, we recorded the electrophysiological responses to quinine, denatonium and naringin usingwhole-cell patch clamp technique in isolated taste cells of C57BL/6J mice. Ten mM quinine induced depolarizing response under the current clamp mode,and inward current response under the voltage-clamp mode (holding potential -80 mV) using both K+ (with pseudo intracellular solution) and Cs+ (K+ was substituted by Cs+ in the pseudo intracellular solution) pipettes, However, when the K+ pipette was used, the membrane conductance was suppressed and activated in succession. On the other hand, the membrane conductance was only activated when the Cs+ pipette was used. Half to one mM denatonium induced depolarizing response under the current clamp mode, and outward current response under the voltage clamp mode with both pipettes, Using these pipettes, the membrane conductance was activated or suppressed in the individual case. Naringin-induced responses were not detected in these measurements. These electrophysiological recordings suggest that multiple transduction mechanisms are involved in bitter taste perception in mouse taste cells.

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Documento generato il 04/04/20 alle ore 21:29:07