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Titolo:
Overexpression of long or short FGFR-1 results in FGF-2-mediated proliferation in neonatal cardiac myocyte cultures
Autore:
Sheikh, F; Fandrich, RR; Kardami, E; Cattini, PA;
Indirizzi:
Univ Manitoba, Dept Physiol, Winnipeg, MB R3E 3J7, Canada Univ Manitoba Winnipeg MB Canada R3E 3J7 ol, Winnipeg, MB R3E 3J7, Canada StSci,iface Gen Hosp, Res Ctr, Dept Human Anat & Cell Sci, Inst CardiovascSt Boniface Gen Hosp Winnipeg MB Canada R2H 2A6 Cell Sci, Inst Cardiovasc
Titolo Testata:
CARDIOVASCULAR RESEARCH
fascicolo: 3, volume: 42, anno: 1999,
pagine: 696 - 705
SICI:
0008-6363(199906)42:3<696:OOLOSF>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
FIBROBLAST GROWTH-FACTOR; FACTOR RECEPTOR-1; HEART DEVELOPMENT; NUCLEAR ACCUMULATION; CELL-PROLIFERATION; FACTOR (FGF)-1; MOUSE HEART; FACTOR-II; EXPRESSION; FAMILY;
Keywords:
myocyte; growth factors; receptors; gene expression; cell culture isolation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Cattini, PA Univ Manitoba, Dept Physiol, 730 William Ave, Winnipeg, MB R3E3J7, Canada Univ Manitoba 730 William Ave Winnipeg MB Canada R3E 3J7 anada
Citazione:
F. Sheikh et al., "Overexpression of long or short FGFR-1 results in FGF-2-mediated proliferation in neonatal cardiac myocyte cultures", CARDIO RES, 42(3), 1999, pp. 696-705

Abstract

Objective: The type 1 fibroblast growth factor receptor (FGFR-1) is the only high affinity receptor for fibroblast growth factor-2 (FGF-2) in the ratmyocardium, and is essential for normal growth and development of the heart. Levels of FGFR-1 are developmentally regulated, being high in embryonic cardiac myocytes. Also, FGFR-1 exists as both 'long' and 'short' isoforms, and there is a switch from predominant expression of the 'long' isoform in the embryo to the 'short' isoform in the adult heart. Both the decrease in receptor levels and the isoform switch in postnatal cardiac myocytes correlate with a loss of proliferative potential. We investigated whether an increase in either 'long' or 'short' FGFR-1 isoforms could stimulate proliferation in postnatal rat cardiac myocyte cultures. Methods and Results: Previously we cloned cDNAs corresponding to 'long' (L) and 'short' (S) FGFR-1 isoforms from embryonic mouse hearts. Hybrid FGFR-1(L) and (S) genes, directed by a myosin light chain-2 promoter and SV40 enhancer sequences, were generated and used to transiently transfect neonatal rat cardiac myocytes. Overexpression of FGFR-1 mRNA and protein was detected by RNA blotting and immunocytochemistry. Ligand-crosslinking confirmed the presence of specific receptors capable of binding FGF-2 on the cell membrane. Overexpression of either FGFR-1(L) or (S) was associated with stimulation of proliferation as assessed by significant increases in bromodeoxyuridine uptake (DNA synthesis) and cell number. To determine whether this response was FGF-2 specific, the level of FGF-2 was assessed in the culture medium of cardiac myocytes overexpressing FGFR-1 isoforms. A three-fold increase was detected in the media of cardiac myocytes overexpressing either FGFR-1(L) or (S) compared to control levels. Neutralization of this FGF-2 with antibodies inhibited the proliferative response. Conclusion: Overexpression of either FGFR-1(L) or (S) resulted in an increase in FGF-2-mediated proliferation of postnatal rat cardiac myocytes. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 04:37:03