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Titolo:
Ryanodine and inositol trisphosphate receptors are differentially distributed and expressed in rat parotid gland
Autore:
Zhang, XJ; Wen, JY; Bidasee, KR; Besch, HR; Wojcikiewicz, RJH; Lee, B; Rubin, RP;
Indirizzi:
SUNY4214falo, Sch Med & Biomed Sci, Dept Pharmacol & Toxicol, Buffalo, NY 1 SUNY Buffalo Buffalo NY USA 14214 Dept Pharmacol & Toxicol, Buffalo, NY 1 Indiana Univ, Sch Med, Dept Pharmacol & Toxicol, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 oxicol, Indianapolis, IN 46202 USA SUNY Hlth Sci Ctr, Dept Pharmacol, Syracuse, NY 13210 USA SUNY Hlth Sci Ctr Syracuse NY USA 13210 Pharmacol, Syracuse, NY 13210 USA
Titolo Testata:
BIOCHEMICAL JOURNAL
, volume: 340, anno: 1999,
parte:, 2
pagine: 519 - 527
SICI:
0264-6021(19990601)340:<519:RAITRA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLIC ADP-RIBOSE; PANCREATIC ACINAR-CELLS; CA2+ RELEASE CHANNELS; 1,4,5-TRISPHOSPHATE RECEPTORS; ADENINE-NUCLEOTIDE; CALCIUM-RELEASE; H-3 RYANODINE; RETICULUM; VESICLES; BINDING;
Keywords:
cyclic ADP-ribose; cytochemical localization; InsP(3) receptor; parotid cell;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Rubin, RP SUNY4214falo, Sch Med & Biomed Sci, Dept Pharmacol & Toxicol, Buffalo, NY 1 SUNY Buffalo Buffalo NY USA 14214 acol & Toxicol, Buffalo, NY 1
Citazione:
X.J. Zhang et al., "Ryanodine and inositol trisphosphate receptors are differentially distributed and expressed in rat parotid gland", BIOCHEM J, 340, 1999, pp. 519-527

Abstract

The present study examines the cellular distribution of the ryanodine receptor/channel (RyR) and inositol 1,4,5-trisphosphate receptor (InsP(3)R) subtypes in parotid acini. Using fluorescently labelled 1,4-difluoro-4-bora-3a,4a-diaza-s-indacene-3-propionic acid glycyl-ryanodine (BODIPY(TM)-ryanodine) and confocal microscopy, RyRs were localized primarily to the perinuclear region (basal pole) of the acinar cell. Ryanodine, Ruthenium Red, cAMP and cADP ribose (cADPR) competed with BODIPY-ryanodine, resulting in a reduction in the fluorescence signal. However, inositol 1,4,5-trisphosphate [Ins(1,4,5)P-3] did not alter the binding of BODIPY-ryanodine. Using receptor-subtype-specific antisera, InsP(3)Rs (types I, II and III) were located predominantly in the apical pole of the parotid cell. The presence of these three subtypes was confirmed using reverse transcriptase PCR with RNA-specific oligonucleotide probes. Binding studies using a parotid cell-membrane fraction identified and characterized RyRs and InsP(3)Rs in terms of binding affinity (K-d) and maximum binding capacity (B-max) and confirmed that cADPR displaces ryanodine from its binding sites. Ruthenium Red and 8-Br-cADP-ribose blocked Ca2+ release in permeabilized acinar cells in response to cADPR and cAMP or forskolin, whereas Ins(1,4,5)P-3-induced Ca2+ release was unaffected. The localization of the RyRs and InsP(3)Rs in discrete regions endowbroad areas of the parotid cell with ligand-activated Ca2+ channels. The consequences of the dual activation of the RyRs and InsP(3)Rs by physiologically relevant stimuli such as noradrenaline (norepinephrine) are consideredin relation to Ca2+ signalling in the parotid gland.

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Documento generato il 13/07/20 alle ore 18:43:42