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Titolo:
High glucose enhances mitogenic response to endothelin-1 in rabbit vascular smooth muscle cells
Autore:
Guo, X; Liu, WL; Yi, FX; Guo, ZG;
Indirizzi:
Hunan Med Univ, Mol Pharmacol Lab, Changsha 410078, Peoples R China Hunan Med Univ Changsha Peoples R China 410078 a 410078, Peoples R China
Titolo Testata:
ACTA PHARMACOLOGICA SINICA
fascicolo: 6, volume: 20, anno: 1999,
pagine: 521 - 524
SICI:
0253-9756(199906)20:6<521:HGEMRT>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
KINASES;
Keywords:
glucose; endothelin-1; Ca2+-calmodulin dependent protein kinase; Western blotting; thoracic aorta; cultured cells; vascular smooth muscle;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Guo, ZG Hunan Med Univ, Mol Pharmacol Lab, Changsha 410078, Peoples R China Hunan Med Univ Changsha Peoples R China 410078 , Peoples R China
Citazione:
X. Guo et al., "High glucose enhances mitogenic response to endothelin-1 in rabbit vascular smooth muscle cells", ACT PHAR SI, 20(6), 1999, pp. 521-524

Abstract

AIM: To examine the effects of high glucose on the mitogenic response of rabbit aortic vascular smooth muscle cells (VSMC) to endothelin-1 (ET-1). METHODS: VSMC were cultured in normal glucose (5.5 mmol . L-1), high glucose (25 mmol . L-1) or high osmolality (glucose 5.5 mmol . L-1, plus mannitol 19.5 mmol L-1). DNA synthesis was measured by [H-3]thymidine incorporation. The expression of phospho-p44/42 MAPK was determined by Western blot. RESULTS: At a concentration range from 10(-12) to 10(-8) mol . LT-1 stimulated [H-3]thymidine incorporation and phospho-p44/42 MAPK expression in VSMC in aconcentration-dependent manner. From 10(-11) to 10(-8) mol . L-1, the mitogenic effect of ET-1 was higher in VSMC cultured in high glucose at equivalent concentration than cells cultured in normal glucose or high osmolality (P < 0.05 or P < 0.01), but no marked difference was observed in the growthresponse between cells cultured under the latter two conditions. Similarly, ET-1 increased expression of phospho-p44/42 MAPK by 60 % - 65 % in VSMC cultured in high glucose, compared with cells in normal glucose or high osmolality. CONCLUSION: VSMC cultured in high glucose exhibited increased mitogenic response to ET-1, which seemed to be related to the enhanced expression of phospho-p44/42 MAPK.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 10:26:01