Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
GRADIENT ELUTION LIQUID-CHROMATOGRAPHY INFRARED SPECTROMETRY AT MU-G 1(-1) LEVEL USING CAPILLARY COLUMN-SWITCHING AND ADDITION OF A MAKE-UPLIQUID - A PRELIMINARY-STUDY
Autore:
VISSER T; VREDENBREGT MJ; TENHOVE GJ; DEJONG APJM; SOMSEN GW;
Indirizzi:
NATL INST PUBL HLTH & ENVIRONM,LOC,POB 1 NL-3720 BA BILTHOVEN NETHERLANDS FREE UNIV AMSTERDAM,DEPT GEN & ANALYT CHEM NL-1081 HV AMSTERDAM NETHERLANDS
Titolo Testata:
Analytica chimica acta
fascicolo: 2-3, volume: 342, anno: 1997,
pagine: 151 - 158
SICI:
0003-2670(1997)342:2-3<151:GELISA>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERFACE; IR;
Keywords:
INFRARED SPECTROMETRY; LIQUID CHROMATOGRAPHY; TRACE ANALYSIS; STRUCTURE ELUCIDATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
T. Visser et al., "GRADIENT ELUTION LIQUID-CHROMATOGRAPHY INFRARED SPECTROMETRY AT MU-G 1(-1) LEVEL USING CAPILLARY COLUMN-SWITCHING AND ADDITION OF A MAKE-UPLIQUID - A PRELIMINARY-STUDY", Analytica chimica acta, 342(2-3), 1997, pp. 151-158

Abstract

The applicability of infrared (CR) spectrometric detection in liquid chromatography (LC), based on solvent-elimination prior to IR scanning, has been enlarged to gradient separations by the adaption of a capillary column switching system and postcolumn addition of a make-up liquid. A pre-column is used for loading and concentration of large samplevolumes. Next, analytes are transferred to the capillary analytical column by a linear gradient flow using methanol as modifier. An excess of methanol (20 mu l min(-1)) is added post-column to the analytical flow (2 mu l min(-1)) which provides the performance of the spray-jet LC-IR interface to become largely independent of the composition of themobile phase. This enables isocratic and gradient elutions with high percentages of water. The potential of the system has been studied with triazines and pyrene as model compounds. Good quality chromatograms and analyte spectra were obtained at the low mg 1(-1) level, using sample Volumes of 40 mu l. If the sample volume was increased to 1 ml, the separation of the test compounds was largely maintained and the identification limits were improved to 10-100 mu g l(-1).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 15:09:17