Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Immunophenotypic characterization of human bone marrow endosteal cells
Autore:
Sillaber, C; Walchshofer, S; Mosberger, I; Gaiger, A; Simonitsch, I; Chott, A; Lechner, K; Valent, P;
Indirizzi:
Univ Vienna, Dept Internal Med 1, Div Hematol & Hemostaseol, A-1090 Vienna, Univ Vienna Vienna Austria A-1090 Hematol & Hemostaseol, A-1090 Vienna, Univ Vienna, Dept Clin Pathol, A-1090 Vienna, Austria Univ Vienna ViennaAustria A-1090 pt Clin Pathol, A-1090 Vienna, Austria
Titolo Testata:
TISSUE ANTIGENS
fascicolo: 6, volume: 53, anno: 1999,
pagine: 559 - 568
SICI:
0001-2815(199906)53:6<559:ICOHBM>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC PROGENITOR CELLS; CHRONIC MYELOID-LEUKEMIA; MONOCLONAL-ANTIBODY; STEM-CELLS; RECEPTOR SUBUNITS; ADHESION MOLECULE; TISSUE-SECTIONS; EXPRESSION; INVITRO; MICROENVIRONMENT;
Keywords:
endosteal cells; stem cells; cytokines; progenitor cell homing; self renewal;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Valent, P Univelienna, Dept Internal Med 1, Div Hematol & Hemostaseol, Wahringer Gurt Univ Vienna Wahringer Gurtel 18-20 Vienna Austria A-1090 r Gurt
Citazione:
C. Sillaber et al., "Immunophenotypic characterization of human bone marrow endosteal cells", TISSUE ANTI, 53(6), 1999, pp. 559-568

Abstract

In order to determine the relationship between bone marrow (bm) endosteal cells (EDC) and hemopoietic progenitors, we have analyzed the immunophenotype of EDC using various antibodies (Ab) against mesenchymal antigens. The Ab were applied on paraffin sections of normal bm (iliac crest, n=17; talus,n=1; phalanx, n=1), myeloregenerative bm (after chemotherapy), and hematologic disorders (acute myeloid leukemia (AML), n=8; chronic myeloid leukemia(CML), n=6; myelodysplastic syndromes (MDS), n=14; severe aplastic anemia (SAA), n=4; essential thrombocythemia (ET), n=2; idiopathic (primary) osteomyelo-fibrosis (IMF), n=1; polycythemia vera (PV), n=1). In normal bm, EDC were found to react with Ab against vimentin, tenascin, alpha-smooth muscleactin, osteocalcin, CD51, and CD56, but did not react with Ab against CD3,CD15, CD20, CD34, CD45, CD68, or CD117. An identical phenotype of EDC was found in AML, MDS, SAA, ET, IMF PV, myeloregenerative bm, and peripheral bones lacking active hemopoiesis (talus, phalanx). In patients with CML, EDC reacted with Ab to CD51, but did not react with Ab to CD56. Based on their unique antigen profile, EDC were enriched from normal bm by enzyme digestion and cell sorting. However, these enriched cells (CD56(+), CD45(-), CD34(-)) did not give rise to hemopoietic cells under the culture conditions used, i.e. in the presence of the growth factors IGF-1, bFGF, SCF, IL-3, and GM-CSF. Together, our data do not support the hypothesis that EDC are totipotent mesenchymal progenitors giving rise to hemopoietic cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 10:32:13