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Titolo:
Induction of the activity of glycolytic enzymes correlates with enhanced hydrolysis of sucrose in the cytosol of transgenic potato tubers
Autore:
Trethewey, RN; Geigenberger, P; Hennig, A; Fleischer-Notter, H; Muller-Rober, B; Willmitzer, L;
Indirizzi:
Max Planck Inst Mol Pflanzenphysiol, D-14476 Golm, Germany Max Planck InstMol Pflanzenphysiol Golm Germany D-14476 6 Golm, Germany Univ Heidelberg, Inst Bot, D-69120 Heidelberg, Germany Univ Heidelberg Heidelberg Germany D-69120 , D-69120 Heidelberg, Germany
Titolo Testata:
PLANT CELL AND ENVIRONMENT
fascicolo: 1, volume: 22, anno: 1999,
pagine: 71 - 79
SICI:
0140-7791(199901)22:1<71:IOTAOG>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
INCREASED ADPGLUCOSE PYROPHOSPHORYLASE; YEAST-DERIVED INVERTASE; SOLANUM-TUBEROSUM; TOBACCO PLANTS; GENE-EXPRESSION; METABOLISM; GLUCOSE; LEADS; CARBOHYDRATE; ACCUMULATION;
Keywords:
ADPglucose pyrophosphorylase; glycolysis; invertase; partitioning; potato tubers; starch metabolism; sugar signalling;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Trethewey, RN MaxGermany Inst Mol Pflanzenphysiol, Karl Liebknecht Str 25,D-14476 Golm, Max Planck Inst Mol Pflanzenphysiol Karl Liebknecht Str 25 Golm Germany D-14476
Citazione:
R.N. Trethewey et al., "Induction of the activity of glycolytic enzymes correlates with enhanced hydrolysis of sucrose in the cytosol of transgenic potato tubers", PL CELL ENV, 22(1), 1999, pp. 71-79

Abstract

The aim of this work was to define the metabolic factors which regulate the respiratory pathways in trangenic potato tubers. We previously found thatrespiration is enhanced in transgenic tubers which express a yeast invertase and a glucokinase from Zymomonas mobilis. In this study we investigated glycolysis in three further transgenic potato lines with profound changes in the mobilization of sucrose. We studied antisense ADPglucose pyrophosphorylase lines which are characterized by a reduction in starch accumulation and a significant build up of sucrose and related metabolic intermediates. We also report the generation of two novel double transgenic lines where theyeast invertase is expressed specifically in tubers of the ADPglucose pyrophosphorylase antisense line, targeted to either the cytosol or apopolast. We evaluated whether the localization of sucrose cleavage had an impact on the glycolytic induction, and assessed if invertase expression in the high-sucrose background had any further effects on glycolysis. We found that induction of the glycolytic enzymes only occurs when the invertase is targetedto the cytosol, and that the extent of this induction was comparable in the wild type and antisenseADPglucose pyrophosphorylase backgrounds. We conclude that the signal regulating glycolysis is directly linked to cytosolic sucrose hydrolysis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 17:02:12