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Titolo:
Dehiscence-related expression of an Arabidopsis thaliana gene encoding a polygalacturonase in transgenic plants of Brassica napus
Autore:
Jenkins, ES; Paul, W; Craze, M; Whitelaw, CA; Weigand, A; Roberts, JA;
Indirizzi:
Univ Nottingham, Sch Biol Sci, Loughborough LE12 5RD, Leics, England Univ Nottingham Loughborough Leics England LE12 5RD 2 5RD, Leics, England Nickerson BIOCEM Ltd, Cambridge CB4 4GZ, England Nickerson BIOCEM Ltd Cambridge England CB4 4GZ ambridge CB4 4GZ, England
Titolo Testata:
PLANT CELL AND ENVIRONMENT
fascicolo: 2, volume: 22, anno: 1999,
pagine: 159 - 167
SICI:
0140-7791(199902)22:2<159:DEOAAT>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
OILSEED RAPE PODS; MESSENGER-RNA; SAMBUCUS-NIGRA; ETHYLENE; ABSCISSION; TOMATO; POLLEN; FRUIT; PURIFICATION; SEQUENCE;
Keywords:
Arabidopsis thaliana; Brassica napus; oilseed rape; pod dehiscence; pod shatter; polygalacturonase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Roberts, JA UnivD,ottingham, Sch Biol Sci, Sutton Bonigton Campus, Loughborough LE12 5R Univ Nottingham Sutton Bonigton Campus Loughborough Leics England LE12 5RD
Citazione:
E.S. Jenkins et al., "Dehiscence-related expression of an Arabidopsis thaliana gene encoding a polygalacturonase in transgenic plants of Brassica napus", PL CELL ENV, 22(2), 1999, pp. 159-167

Abstract

Pod dehiscence in Arabidopsis thaliana is accompanied by an increase in the expression of a polygalacturonase (PG). The gene encoding this mRNA has been characterized and shown to have extensive homology to a similar PG genefrom Brassica napus. The A. thaliana PG promoter was fused to beta-glucuronidase (GUS) and the expression of this reporter gene analysed in transgenic B. napus plants. The GUS activity was detected throughout the dehiscence zone of pods from 35 d after anthesis and expression was restricted to those cells that undergo cell separation. Expression was also detectable at thejunction between the seed and the funicular tissue and in mature anthers of flowers. Transgenic plants containing the PG promoter fused to barnase were sterile as a consequence of the anthers failing to undergo dehiscence. Fertilization of PG barnase plants resulted in the development of pods that exhibited a reduced capacity to shatter. The role of PG during cell separation processes in plants is discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 20:40:11