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Titolo:
Simple stereological procedure to estimate the number and dimensions of root hairs
Autore:
Wulfsohn, D; Nyengaard, JR;
Indirizzi:
Univdaaskatchewan, Dept Agr & Bioresource Engn, Saskatoon, SK S7N 5A9, Cana Univ Saskatchewan Saskatoon SK Canada S7N 5A9 Saskatoon, SK S7N 5A9, Cana Aarhus Univ, Stereol Res Lab, DK-8000 Aarhus C, Denmark Aarhus Univ Aarhus Denmark C Stereol Res Lab, DK-8000 Aarhus C, Denmark
Titolo Testata:
PLANT AND SOIL
fascicolo: 1, volume: 209, anno: 1999,
pagine: 129 - 136
SICI:
0032-079X(1999)209:1<129:SSPTET>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARBITRARY PARTICLES; EFFICIENCY;
Keywords:
Agropyron cristatum L.; diameter; disector; length; surface area; systematic sampling; variability;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Wulfsohn, D Univ Saskatchewan, Dept Agr & Bioresource Engn, 57 Campus Dr, Saskatoon, SK Univ Saskatchewan 57 Campus Dr Saskatoon SK Canada S7N 5A9 SK
Citazione:
D. Wulfsohn e J.R. Nyengaard, "Simple stereological procedure to estimate the number and dimensions of root hairs", PLANT SOIL, 209(1), 1999, pp. 129-136

Abstract

We demonstrate a simple procedure for systematic, uniformly random sampling of a root system of known length to obtain practically unbiased estimatesof the total number and dimensions of root hairs. Irrespective of the length of the root system, only 100 root hairs need to be counted to estimate the total number with sufficient precision. Numbers and dimensions of root hairs were estimated for five crested wheatgrass (Agropyron cristatum L.) root systems that had been grown for one month in a gel. Less than one hour was required to obtain estimates of root hair parameters for a single plant. There was low variability of spatial density of root hairs within a given branching order (CV < 15%); however, because of large variation in the total length of laterals, the total number of root hairs varied greatly (CV similar to 70%). On average, root hairs provided half of the total surface area of a root system and a total length 20 times that of the roots.

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Documento generato il 04/12/20 alle ore 18:28:09