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Titolo:
The multiply-regulated gabA gene encoding the GABA permease of Aspergillusnidulans: a score of exons
Autore:
Hutchings, H; Stahmann, KP; Roels, S; Espeso, EA; Timberlake, WE; Arst, HN; Tilburn, J;
Indirizzi:
HammersmithEnglandImperial Coll, Sch Med, Dept Infect Dis, London W12 0NN,Hammersmith Hosp London England W12 0NN Dept Infect Dis, London W12 0NN, Millennium Pharmaceut, Cambridge, MA 02139 USA Millennium Pharmaceut Cambridge MA USA 02139 eut, Cambridge, MA 02139 USA
Titolo Testata:
MOLECULAR MICROBIOLOGY
fascicolo: 3, volume: 32, anno: 1999,
pagine: 557 - 568
SICI:
0950-382X(199905)32:3<557:TMGGET>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARBON CATABOLITE REPRESSION; FINGER TRANSCRIPTION FACTOR; NITROGEN METABOLITE REPRESSION; MEMBRANE-SPANNING PROTEINS; AMBIENT PH; ZINC-FINGER; NUCLEOTIDE-SEQUENCE; BINDING-SITES; 4-AMINOBUTYRATE TRANSPORTER; INTEGRATOR GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
71
Recensione:
Indirizzi per estratti:
Indirizzo: Tilburn, J HammersmithnHosp, Imperial Coll, Sch Med, Dept Infect Dis, Ducane Rd, Londo Hammersmith Hosp Ducane Rd London England W12 0NN ne Rd, Londo
Citazione:
H. Hutchings et al., "The multiply-regulated gabA gene encoding the GABA permease of Aspergillusnidulans: a score of exons", MOL MICROB, 32(3), 1999, pp. 557-568

Abstract

We describe the cloning, sequence and expression of gabA, encoding the gamma-amino-n-butyrate (GABA) permease of the fungus Aspergillus nidulans, Sequence changes were determined for three up-promoter (gabI) and six gabA loss-of-function mutations. The predicted protein contains 517 residues and shows 30.3% overall identity with a putative GABA permease of Arabidopsis thaliana, 29.6% identity with the yeast choline transporter and 23.4% identitywith the yeast UGA4 GABA permease. Structural predictions favour 11-12 transmembrane domains. Comparison of the genomic and cDNA sequences shows the presence of 19 introns, an unusually large number of introns for, we believe, any fungal gene. In agreement with the wealth of genetic data available,transcript level analyses demonstrate that gabA is subject to carbon catabolite and nitrogen metabolite repression, omega-amino acid induction and regulation in response to ambient pH (being acid-expressed). In agreement with this, we report consensus binding sites 5' to the coding region, six eachfor CreA and AREA and one for PacC, the transcription factors mediating carbon catabolite and nitrogen metabolite repression and response to ambient pH respectively.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 21:14:21