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Titolo:
A one-tube nucleic acid extraction and amplification (ERTPCR) method for detecting RNA viruses
Autore:
Cook, N; Kurdziel, AS; Hays, JP; Gordon, SH; Donald, BJ; Myint, SH;
Indirizzi:
MinistZ,gr Fisheries & Food, Cent Sci Lab, Food Microbiol Grp, York YO41 1L Minist Agr Fisheries & Food York N Yorkshire England YO41 1LZ ork YO41 1L Univ Leicester, Dept Microbiol & Immunol, Leicester LE1 4HN, Leics, England Univ Leicester Leicester Leics England LE1 4HN er LE1 4HN, Leics, England
Titolo Testata:
JOURNAL OF RAPID METHODS AND AUTOMATION IN MICROBIOLOGY
fascicolo: 1, volume: 7, anno: 1999,
pagine: 61 - 68
SICI:
1060-3999(199905)7:1<61:AONAEA>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEPATITIS-A VIRUS; POLYMERASE CHAIN-REACTION; ENVIRONMENTAL-SAMPLES; PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Cook, N MinistZ,gr Fisheries & Food, Cent Sci Lab, Food Microbiol Grp, York YO41 1L Minist Agr Fisheries & Food York N Yorkshire England YO41 1LZ 1L
Citazione:
N. Cook et al., "A one-tube nucleic acid extraction and amplification (ERTPCR) method for detecting RNA viruses", J RAPID M A, 7(1), 1999, pp. 61-68

Abstract

Extraction Reverse Transcription Polymerase Chain Reaction (ERTPCR), a rapid and simple method which allows detection of RNA viruses, is described. Unlike conventional Reverse Transcription Polymerase Chain Reaction(RTPCR), which requires extraction of viral nucleic acid prior to reverse transcription and cDNA amplification, ERTPCR allows these procedures to be accomplished in one step in the same reaction tube. This is facilitated through heat extraction of viral RNA, and the use of a thermostable enzyme which can perform reverse transcription and cDNA amplification in the same buffer system. The technique is at least as sensitive as a standard RTPCR. Its use in detecting rotavirus in feces, and hepatitis A virus in infected tissue cultures, is demonstrated. A silica spin column was used to remove RTPCR inhibitors from these samples.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/10/20 alle ore 17:41:39