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Titolo:
A novel immunodeficient mouse model - RAG2 x common cytokine receptor gamma chain double mutants - Requiring exogenous cytokine administration for human hematopoietic stem cell engraftment
Autore:
Mazurier, F; Fontanellas, A; Salesse, S; Taine, L; Landriau, S; Moreau-Gaudry, F; Reiffers, J; Peault, B; Di Santo, JP; de Verneuil, H;
Indirizzi:
Univ Bordeaux 2, Lab Pathol & Therapie Gen, F-33076 Bordeaux, France Univ Bordeaux 2 Bordeaux France F-33076 ie Gen, F-33076 Bordeaux, France Univ5540,eaux 2, Federat Rech Biol Greffes 60, Lab Greffe Moelle, CNRS,UMRUniv Bordeaux 2 Bordeaux France F-33076 60, Lab Greffe Moelle, CNRS,UMR Inst Bergonie, Dept Radiotherapie & Radiophys, Bordeaux, France Inst Bergonie Bordeaux France diotherapie & Radiophys, Bordeaux, France Coll France, CNRS, UMR 9924, Nogent Sur Marne, France Coll France Nogent Sur Marne France UMR 9924, Nogent Sur Marne, France CNRS, Inst Embryol Cellulaire & Mol, F-94736 Nogent Sur Marne, France CNRS Nogent Sur Marne France F-94736 l, F-94736 Nogent Sur Marne, France Hop Necker Enfants Malad, INSERM, U429, Paris, France Hop Necker Enfants Malad Paris France alad, INSERM, U429, Paris, France
Titolo Testata:
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
fascicolo: 5, volume: 19, anno: 1999,
pagine: 533 - 541
SICI:
1079-9907(199905)19:5<533:ANIMM->2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
NATURAL-KILLER-CELLS; SCID-HU MOUSE; BONE-MARROW; GENE-THERAPY; LYMPHOID DEVELOPMENT; PERIPHERAL-BLOOD; MICE DEFICIENT; T-CELLS; LYMPHOCYTES; TRANSPLANTATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: de Verneuil, H Univrdeaux,ux 2, Lab Pathol & Therapie Gen, 146 Rue Leo Saignat, F-33076 Bo Univ Bordeaux 2 146 Rue Leo Saignat Bordeaux France F-33076
Citazione:
F. Mazurier et al., "A novel immunodeficient mouse model - RAG2 x common cytokine receptor gamma chain double mutants - Requiring exogenous cytokine administration for human hematopoietic stem cell engraftment", J INTERF CY, 19(5), 1999, pp. 533-541

Abstract

Gene transduction into immature human hematopoietic cells collected from umbilical cord blood, bone marrow, or mobilized peripheral blood cells couldbe useful for the treatment of genetic and acquired disorders of the hematopoietic system, Immunodeficient mouse models have been used frequently as recipients to assay the growth and differentiation of human hematopoietic stem/progenitor cells. Indeed, high levels of human cell engraftment were first reported in human/murine chimeras using NOD/SCID mice, which now are considered as the standard for these types of experiments. However, NOD/SCID mice have some clear disadvantages (including spontaneous tumor formation) that limit their general use. We have developed a new immunodeficient mousemodel by combining recombinase activating gene-2 (RAG2) and common cytokine receptor gamma chain (gamma c) mutations. The RAG2(-/-)/gamma c(-) doublemutant mice are completely alymphoid (T-, B-, NK-), show no spontaneous tumor formation, and exhibit normal hematopoietic parameters. Interestingly, human cord blood cell engraftment in RAG2(-/-)/gamma c(-) mice was greatly enhanced by the exogenous administration of human cytokines interleukin-(IL-3) granulocyte-macrophage colony-stimulating factor, (GM-CSF), and erythropoietin in contrast to the NOD/SCID model. This unique feature of the RAG2(-/-)/gamma c(-) mouse model should be particularly well suited for assessing the role of different cytokines in human lymphopoiesis and stem/progenitor cell function in vivo.

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Documento generato il 30/10/20 alle ore 09:32:43