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Titolo:
IFN-beta interferes with the differentiation of dendritic cells from peripheral blood mononuclear cells: Selective inhibition of CD40-dependent interleukin-12 secretion
Autore:
Bartholome, EJ; Willems, F; Crusiaux, A; Thielemans, K; Schandene, L; Goldman, M;
Indirizzi:
Hop Erasme, Dept Immunol, B-1070 Brussels, Belgium Hop Erasme Brussels Belgium B-1070 ept Immunol, B-1070 Brussels, Belgium Free Univ Brussels, Ctr Rech Interuniv Vaccinol, Brussels, Belgium Free Univ Brussels Brussels Belgium teruniv Vaccinol, Brussels, Belgium Free Univ Brussels, Lab Physiol Immunol, Brussels, Belgium Free Univ Brussels Brussels Belgium Physiol Immunol, Brussels, Belgium
Titolo Testata:
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
fascicolo: 5, volume: 19, anno: 1999,
pagine: 471 - 478
SICI:
1079-9907(199905)19:5<471:IIWTDO>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; CD4(+) T-CELLS; EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS; FACTOR-KAPPA-B; MULTIPLE-SCLEROSIS; CLASS-II; GAMMA PRODUCTION; COSTIMULATORY MOLECULES; ANTIGEN EXPRESSION; IMMUNE-RESPONSES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Goldman, M Hop Erasme, Dept Immunol, Route Lennik 808, B-1070 Brussels, Belgium Hop Erasme Route Lennik 808 Brussels Belgium B-1070 s, Belgium
Citazione:
E.J. Bartholome et al., "IFN-beta interferes with the differentiation of dendritic cells from peripheral blood mononuclear cells: Selective inhibition of CD40-dependent interleukin-12 secretion", J INTERF CY, 19(5), 1999, pp. 471-478

Abstract

We studied the effects of interferon-beta (IFN-beta) on the differentiation of dendritic cells (DC) obtained by culturing plastic-adherent peripheralblood mononuclear cells (PBMC) from a total of 30 healthy volunteers in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) andinterleukin-4 (IL-4). First, we found that the addition of IFN-beta at theinitiation of the culture did not modify DC morphology but caused a reproducible and statistically significant upregulation of HLA-DR, CD86, and CD80surface expression. CD1a expression was significantly reduced, and CD40 expression was unchanged. We then determined the influence of IFN-beta on theproduction of cytokines by DC. DC differentiated in the presence of IFN-beta secreted significantly less IL-12 (p40 and p70) both spontaneously and on activation by fibroblasts transfected with the CD40L gene. This effect ofIFN-beta was dose dependent and selective, as it was not observed for IL-6, IL-8, and tumor necrosis factor-alpha (TNF-alpha). As a consequence, DC differentiated in the presence of IFN-beta induced significantly less IFN-gamma secretion by alloreactive T cells, whereas they were more efficient than control DC in eliciting IL-5 secretion. We conclude that the direct action of IFN-beta on DC causes inhibition of their ability to secrete IL-12 in response to CD40 ligation and to elicit Th1 type responses.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/08/20 alle ore 17:05:05