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Titolo:
CD3-mediated T-cell activation is inhibited by anti-CD44 monoclonal antibodies directed to the hyaluronan-binding region
Autore:
Sugiyama, K; Komada, Y; Deguchi, T; Zhang, XL; Azuma, E; Ido, M; Yamamoto, H; Sakurai, M;
Indirizzi:
Mie Univ, Sch Med, Dept Pediat, Tsu, Mie 5148507, Japan Mie Univ Tsu Mie Japan 5148507 Med, Dept Pediat, Tsu, Mie 5148507, Japan Mie Univ, Sch Med, Dept Clin Immunol, Tsu, Mie 5148507, Japan Mie Univ Tsu Mie Japan 5148507 Dept Clin Immunol, Tsu, Mie 5148507, Japan Mie Univ, Sch Med, Dept Mol Pathobiol, Tsu, Mie 5148507, Japan Mie Univ Tsu Mie Japan 5148507 ept Mol Pathobiol, Tsu, Mie 5148507, Japan Natl Mie Chuo Hosp, Dept Pediat, Hisai, Mie 5141101, Japan Natl Mie Chuo Hosp Hisai Mie Japan 5141101 iat, Hisai, Mie 5141101, Japan
Titolo Testata:
IMMUNOLOGICAL INVESTIGATIONS
fascicolo: 2-3, volume: 28, anno: 1999,
pagine: 185 - 200
SICI:
0882-0139(1999)28:2-3<185:CTAIIB>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERIPHERAL-BLOOD LYMPHOCYTES; CYTOPLASMIC FREE CALCIUM; TYROSINE KINASES; CD44; RECEPTOR; MOLECULE; RECOGNITION; ADHESION; PROTEIN; PATHWAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Komada, Y Mie Univ, Sch Med, Dept Pediat, 2-174 Edobashi, Tsu, Mie 5148507, Japan Mie Univ 2-174 Edobashi Tsu Mie Japan 5148507 Mie 5148507, Japan
Citazione:
K. Sugiyama et al., "CD3-mediated T-cell activation is inhibited by anti-CD44 monoclonal antibodies directed to the hyaluronan-binding region", IMMUNOL INV, 28(2-3), 1999, pp. 185-200

Abstract

The CD44 molecule has been shown to play a role in T cell adhesion and activation. We have investigated the ability of five anti-CD44 monoclonal antibodies (MoAb) including 15C6, 18A3, BU75 (Ancell), J173 (Immunotech), and L178 (Becton Dickinson) to regulate T cell activation. Three MoAb: 15C6, BU75, and J173 were found to selectively inhibit DNA synthesis, interleukin-2 (IL-2) receptor expression, and G1-->S transition of the cell cycle in T cells stimulated with anti-CD3 MoAb. None of anti-CD44 MoAb had influence on T cell proliferation induced by IL-2 or phorbol 12-myristate 13-acetate plus ionomycin, Inhibition of the CD3 pathway by anti-CD44 MoAb occurred by binding of MoAb directly to T cells without the involvement of monocytes or Fe receptors. In addition, the inhibitory anti-CD44 MoAb clearly suppressed intracellular calcium mobilization in T cells stimulated with anti-CD3 MoAb. Interestingly, the ability of anti-CD44 MoAb to inhibit T cell activationwas well correlated with their capability to block the binding of hyaluronan (HA) to CD44 molecules. These results suggest that anti-CD44 MoAb directed to HA-binding site could selectively inhibit CD3-mediated T cell activation. Furthermore, CD44-mediated inhibitory signals would be linked to the blocking of early CD3-mediated signal transduction.

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Documento generato il 05/04/20 alle ore 18:42:07