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Titolo:
Kinin and histamine stimulate Cl- secretion in gerbil middle ear epithelium: connection to otitis media
Autore:
Furukawa, M; Suzuki, H; Ikeda, K; Oshima, T; Yamaya, M; Sasaki, H; Takasaka, T;
Indirizzi:
Tohoku4,niv, Sch Med, Dept Otorhinolaryngol, Aoba Ku, Sendai, Miyagi 980857 Tohoku Univ Sendai Miyagi Japan 9808574 l, Aoba Ku, Sendai, Miyagi 980857 Tohokupaniv, Sch Med, Dept Geriatr Med, Aoba Ku, Sendai, Miyagi 9808574, Ja Tohoku Univ Sendai Miyagi Japan 9808574 ba Ku, Sendai, Miyagi 9808574, Ja Sendai0,atl Hosp, Dept Otorhinolaryngol, Miyagino Ku, Sendai, Miyagi 983852 Sendai Natl Hosp Sendai Miyagi Japan 9838520 no Ku, Sendai, Miyagi 983852
Titolo Testata:
HEARING RESEARCH
fascicolo: 1-2, volume: 132, anno: 1999,
pagine: 109 - 116
SICI:
0378-5955(199906)132:1-2<109:KAHSCS>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
INFLAMMATORY MEDIATORS; EXPRESSION CLONING; RECEPTOR; BRADYKININ; EFFUSION; RAT; MEMBRANES; RESPONSES;
Keywords:
short-circuit current; intracellular Ca2+ concentration; Lysyl-bradykinin; H-1 histamine receptor; H-1 bradykinin receptor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Furukawa, M Tohokudai,v, Sch Med, Dept Otorhinolaryngol, Aoba Ku, 1-1 Seiryo Machi, Sen Tohoku Univ 1-1 Seiryo Machi Sendai Miyagi Japan 9808574 , Sen
Citazione:
M. Furukawa et al., "Kinin and histamine stimulate Cl- secretion in gerbil middle ear epithelium: connection to otitis media", HEARING RES, 132(1-2), 1999, pp. 109-116

Abstract

The effects of bradykinin (BK) and histamine on transepithelial ion transport in primary cultures of gerbil middle ear epithelium were investigated. Lysyl-bradykinin (lys-BK) elicited a transient increase in short-circuit current (I-sc) when added to apical or basolateral surfaces. Lys-BK had a larger effect than BK or des-arg(9)-BK on both epithelial surfaces. Histamine induced a transient increase in I-sc. only when added to the basolateral surface. Mepyramine, an H-1 histamine antagonist, greatly reduced the histamine-induced I-sc. The H-2 and H-3 histamine antagonists were both ineffective for inhibiting the I-sc responses to histamine. Diphenylamine-2-carboxylate or 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid, Cl- channel blockers, significantly blocked the I-sc responses to lys-BK or histamine. The Ca2-mobilizing action of lys-BK and histamine was also investigated in singlemiddle ear epithelial cells. BK and histamine induced an increase in the intracellular Ca2+ concentration. 1,2-Bis-(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid-acetoxymethyl ester, a calcium chelator, greatly reduced the increase in the I-sc responses to lys-BK or histamine. These data indicate that BK and histamine activate intracellular Ca2+-dependent mechanisms, leading to apical Cl- secretion in the cultured gerbil middle ear epitheliumvia B-2 BK receptors and H-1 histamine receptors. (C) 1999 Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 06:54:01