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Titolo:
Cryopreservation of in vitro-grown shoot tips of banana (Musa spp) by vitrification method
Autore:
Thinh, NT; Takagi, H; Yashima, S;
Indirizzi:
Japanannt Res Ctr Agr Sci, Okinawa Sub Trop Stn, Ishigaki, Okinawa 907, Jap Japan Int Res Ctr Agr Sci Ishigaki Okinawa Japan 907 ki, Okinawa 907, Jap
Titolo Testata:
CRYO-LETTERS
fascicolo: 3, volume: 20, anno: 1999,
pagine: 163 - 174
SICI:
0143-2044(199905/06)20:3<163:COIVST>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
WASABI WASABIA-JAPONICA; APICAL MERISTEMS; PLANT-REGENERATION; CELLS; DEHYDRATION; CULTURES;
Keywords:
cryopreservation; vitrification; Musa spp; shoot tip;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Thinh, NT Japani,nt Res Ctr Agr Sci, Okinawa Sub Trop Stn, 1091-1 Kawarabaru, Ishigak Japan Int Res Ctr Agr Sci 1091-1 Kawarabaru Ishigaki Okinawa Japan 907
Citazione:
N.T. Thinh et al., "Cryopreservation of in vitro-grown shoot tips of banana (Musa spp) by vitrification method", CRYO-LETT, 20(3), 1999, pp. 163-174

Abstract

Vitrification was successfully applied to the cryopreservation of shoot tips isolated from in vitro plantlets of different cultivars of banana. Threeessential conditions were identified to optimize the protocol i.e. 1) selection of suitable shoot tips as explants, 2) loading with a solution of 2M glycerol + 0.4M sucrose for 20 min at 25 degrees C and 3) dehydration with a vitrification solution (PVS2) for 20-30 min at 0 degrees C before rapid plunging of the shoot tips into liquid nitrogen. Compared to other cryostorage techniques for banana germplasm, this protocol appeared to be simpler toconduct and resulted in higher rates of post-thaw recovery (average, 69%).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 21:55:33