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Titolo:
Genetic variation between strains of Monilinia fructicola and Monilinia laxa isolated from cherries in Michigan
Autore:
Snyder, CL; Jones, AL;
Indirizzi:
Michigan State Univ, Dept Bot & Plant Pathol, E Lansing, MI 48824 USA Michigan State Univ E Lansing MI USA 48824 athol, E Lansing, MI 48824 USA
Titolo Testata:
CANADIAN JOURNAL OF PLANT PATHOLOGY-REVUE CANADIENNE DE PHYTOPATHOLOGIE
fascicolo: 1, volume: 21, anno: 1999,
pagine: 70 - 77
SICI:
0706-0661(199903)21:1<70:GVBSOM>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROUP-I INTRON; RIBOSOMAL-SUBUNIT RNA; FIELD POPULATIONS; ARBITRARY PRIMERS; DNA; IDENTIFICATION; SEQUENCES; FUNGI; SCLEROTINIACEAE; AMPLIFICATION;
Keywords:
18S rDNA; American brown rot; European brown rot;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Jones, AL Michigan State Univ, Dept Bot & Plant Pathol, E Lansing, MI 48824 USA Michigan State Univ E Lansing MI USA 48824 ansing, MI 48824 USA
Citazione:
C.L. Snyder e A.L. Jones, "Genetic variation between strains of Monilinia fructicola and Monilinia laxa isolated from cherries in Michigan", CAN J PL P, 21(1), 1999, pp. 70-77

Abstract

Polymerase chain reaction (PCR)-mediated analysis of rDNA from isolates ofMonilinia fructicola and Monilinia laxa from Michigan cherry orchards revealed interspecies restriction site variation in the internal transcribed spacer 1 (ITS1) region and length variation in the small subunit (SSU) rRNA gene. ITS1 sequences from both species were 146 bp long; however, the ITS1 of M. laxa differed at three positions from the ITS 1 of M. fructicola. Although the sequences of the ITS1 regions from both species were nearly identical, the enzyme MseI cuts the PCR-amplified ITS1 region of the two species differentially. PCR amplification of the 3' end of the SSU rRNA gene yielded products of approximately 940 and 520 bp from M. fructicola and IM. lasa,respectively. A 421-bp group I intron was detected by PCR within the SSU rDNA of 32 isolates of M. fructicola but not in the eight isolates of M, laxa. Intron sequences from each of four isolates of M. fructicola were identical, and the SSU rDNA nanking sequences from these isolates and from two isolates of M. laxa were nearly identical. Arbitrarily primed PCR analysis ofgenomic DNA with microsatellite primers (GACA)(4) and (GTG)(5) revealed that the number and size of the amplification products were characteristic for each species. Distinctive and reproducible sets of amplification productswere obtained from 32 isolates of M. fructicola and the eight isolates of M. laxa. Our results illustrate the potential of PCR amplification of ribosomal and genomic DNA for differentiating these tree-fruit-infecting brown rot fungi.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/04/20 alle ore 02:33:16