Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
IL-2-deficient mice raised under germfree conditions develop delayed mild focal intestinal inflammation
Autore:
Schultz, M; Tonkonogy, SL; Sellon, RK; Veltkamp, C; Godfrey, VL; Kwon, J; Grenther, WB; Balish, E; Horak, I; Sartor, RB;
Indirizzi:
UnivestCarolina, Sch Med, Dept Med,Ctr Gastrointestinal Biol & Dis, Div Dig Univ N Carolina Chapel Hill NC USA 27599 rointestinal Biol & Dis, Div Dig Univ N Carolina, Sch Med, Dept Pathol & Lab Med, Chapel Hill, NC 27599 USAUniv N Carolina Chapel Hill NC USA 27599 b Med, Chapel Hill, NC 27599 USA N Carolina State Univ, Coll Vet Med, Raleigh, NC 27606 USA N Carolina State Univ Raleigh NC USA 27606 Vet Med, Raleigh, NC 27606 USA Univ Wisconsin, Dept Surg, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 sin, Dept Surg, Madison, WI 53706 USA Univ Wisconsin, Dept Med Microbiol, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 Med Microbiol, Madison, WI 53706 USA Univ Wisconsin, Dept Immunol, Madison, WI 53706 USA Univ Wisconsin Madison WI USA 53706 , Dept Immunol, Madison, WI 53706 USA Inst Mol Pharmacol, Dept Mol Genet, D-12207 Berlin, Germany Inst Mol Pharmacol Berlin Germany D-12207 Genet, D-12207 Berlin, Germany
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
fascicolo: 6, volume: 39, anno: 1999,
pagine: G1461 - G1472
SICI:
0193-1857(199906)39:6<G1461:IMRUGC>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERLEUKIN-2 DEFICIENT MICE; IMMUNE-SYSTEM ACTIVATION; HLA-B27 TRANSGENIC RATS; T-CELLS; INTERLEUKIN-10-DEFICIENT MICE; EXPERIMENTAL COLITIS; BACTERIAL OVERGROWTH; LYMPHOCYTE-RESPONSES; BOWEL INFLAMMATION; ULCERATIVE-COLITIS;
Keywords:
interleukin-2-deficient mice; luminal flora; colitis; animal models;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Sartor, RB UnivestCarolina, Sch Med, Dept Med,Ctr Gastrointestinal Biol & Dis, Div Dig Univ N Carolina CB 7080,Rm 030,Glaxo Bldg Chapel Hill NC USA 27599
Citazione:
M. Schultz et al., "IL-2-deficient mice raised under germfree conditions develop delayed mild focal intestinal inflammation", AM J P-GAST, 39(6), 1999, pp. G1461-G1472

Abstract

Interleukin-2 (IL-2) amplifies immune stimuli and influences B cell differentiation. IL-2-deficient mice spontaneously develop intestinal inflammation if raised under specific pathogen-free (SPF) conditions. We quantitatively determined the aggressiveness and kinetics of gastrointestinal and hepatic inflammation in the presence or absence of viable bacteria in IL-2-deficient mice. Breeding colonies were maintained under SPF and germfree (GF) conditions. Intestinal tissues, serum, and mesenteric lymph nodes were obtained from mice at different ages for blind histological scoring, immunoglobulin measurements, mucosal T cell infiltration, and cytokine secretion. GF IL-2 -/- mice developed mild, focal, and nonlethal intestinal inflammation with delayed onset, whereas the more aggressive inflammation in SPF IL-2 -/- mice led to their death between 28 and 32 wk. Periportal hepatic inflammation was equal in the presence or absence of bacterial colonization. Intestinal immunoglobulin secretion decreased significantly by 13 wk of age in IL-2 -/- mice in both GF and SPF environments. In contrast to other genetically engineered rodents, IL-2 -/- mice develop mild focal gastrointestinal and active portal tract inflammation in the absence of viable bacteria.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 11:04:23