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Titolo:
Metabolic acidosis regulates rat renal Na-S-i cotransport activity
Autore:
Puttaparthi, K; Markovich, D; Halaihel, N; Wilson, P; Zajicek, HK; Wang, HM; Biber, J; Murer, H; Rogers, T; Levi, M;
Indirizzi:
Dept Vet Affairs Med Ctr, Dallas, TX 75216 USA Dept Vet Affairs Med Ctr Dallas TX USA 75216 ed Ctr, Dallas, TX 75216 USA Univ Zurich, Inst Physiol, CH-8057 Zurich, Switzerland Univ Zurich Zurich Switzerland CH-8057 siol, CH-8057 Zurich, Switzerland Univ Queensland, Dept Physiol & Pharmacol, Brisbane, Qld 4072, Australia Univ Queensland Brisbane Qld Australia 4072 Brisbane, Qld 4072, Australia
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 6, volume: 45, anno: 1999,
pagine: C1398 - C1404
SICI:
0363-6143(199906)45:6<C1398:MARRRN>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
BRUSH-BORDER VESICLES; INORGANIC SULFATE; PHOSPHATE-TRANSPORT; EXPRESSION CLONING; DIETARY SULFATE; MESSENGER-RNA; THYROID-HORMONE; H+ EXCHANGE; ADAPTATION; ANTIPORTER;
Keywords:
sodium-inorganic sulfate cotransport; brush-border membrane; serum sulfate; urinary excretion; Xenopus laevis oocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Levi, M 4500 S Lancaster Rd,MC 151, Dallas, TX 75216 USA 4500 S LancasterRd,MC 151 Dallas TX USA 75216 llas, TX 75216 USA
Citazione:
K. Puttaparthi et al., "Metabolic acidosis regulates rat renal Na-S-i cotransport activity", AM J P-CELL, 45(6), 1999, pp. C1398-C1404

Abstract

Recently, me cloned a cDNA (NaSi-1) localized to rat renal proximal tubules and encoding the brush-border membrane (BBM) Na gradient-dependent inorganic sulfate (S-i) transport protein (Na-S-i cotransporter). The purpose of the present study was to determine the effect of metabolic acidosis (MA) onNa-S-i cotransport activity and NaSi-1 protein and mRNA expression. In rats with MA for 24 h (but not 6 or 12 h), there was a significant increase inthe fractional excretion of S-i, which was associated with a 2,4-fold decrease in BBM Na-S-i cotransport activity. The decrease in Na-S-i cotransportcorrelated with a 2.8-fold decrease in BBM NaSi-1 protein abundance and a 2.2-fold decrease in cortical NaSi-1 mRNA abundance. The inhibitory effect of MA on BBM Na-Si cotransport was also sustained in rats with chronic (10 days) MA. In addition, in Xenopus laevis oocytes injected with mRNA from kidney cortex, there was a significant reduction in the induced Na-S-i cotransport in rats with MA compared with control rats, suggesting that MA causesa decrease in the abundance of functional mRNA encoding the NaSi-1 cotransporter. These findings indicate that MA reduces Si reabsorption by causing decreases in BBM Na-S-i cotransport activity and that decreases in the expression of NaSi-1 protein and mRNA abundance, at least in part, play an important role in the inhibition of Na-S-i cotransport activity during MA.

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Documento generato il 10/07/20 alle ore 19:03:21