Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Potyvirus helper component-proteinase self-interaction in the yeast two-hybrid system and delineation of the interaction domain involved
Autore:
Urcuqui-Inchima, S; Walter, J; Drugeon, G; German-Retana, S; Haenni, AL; Candresse, T; Bernardi, F; Le Gall, O;
Indirizzi:
Inst Jacques Monod, F-75251 Paris 05, France Inst Jacques Monod Paris France 05 cques Monod, F-75251 Paris 05, France INRA, F-33883 Villenave Dornon, France INRA Villenave Dornon France F-33883 A, F-33883 Villenave Dornon, France
Titolo Testata:
VIROLOGY
fascicolo: 1, volume: 258, anno: 1999,
pagine: 95 - 99
SICI:
0042-6822(19990525)258:1<95:PHCSIT>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACID-BINDING PROPERTIES; HC-PRO GENE; GENOME AMPLIFICATION; MUTATIONAL ANALYSIS; APHID TRANSMISSION; MOSAIC POTYVIRUS; TOBACCO; SUPPRESSION; EXPRESSION; VIRUSES;
Keywords:
HC-Pro; potyvirus; dimer formation; two-hybrid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Walter, J Inst Jacques Monod, 2 Pl Jussieu,Tour 43, F-75251 Paris 05, France Inst Jacques Monod 2 Pl Jussieu,Tour 43 Paris France 05 France
Citazione:
S. Urcuqui-Inchima et al., "Potyvirus helper component-proteinase self-interaction in the yeast two-hybrid system and delineation of the interaction domain involved", VIROLOGY, 258(1), 1999, pp. 95-99

Abstract

Using the yeast two-hybrid system, a screen was performed for possible interactions between the proteins encoded by the 5' region of potyviral genomes [P1, helper component-proteinase (HC-Pro), and P3]. A positive self-interaction involving HC-Pro was detected with lettuce mosaic virus (LMV) and potato virus Y (PVY). The possibility of heterologous interaction between theHC-Pro of LMV and of PW was also demonstrated. No interaction involving either the P1 or the P3 proteins was detected. A series of ordered deletions from either the N- or C-terminal end of the LMV HC-Pro was used to map the domain involved in interaction to the 72 N-terminal amino acids of the protein, a region known to be dispensable for virus viability but necessary foraphid transmission. A similar but less detailed analysis mapped the interacting domain to the N-terminal half of the PW HC-Pro. (C) 1999 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 10:31:04