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Titolo:
BUNDLE SHEATH DEFECTIVE2, a novel protein required for post-translational regulation of the rbcL gene of maize
Autore:
Brutnell, TP; Sawers, RJH; Mant, A; Langdale, JA;
Indirizzi:
Univ Oxford, Dept Plant Sci, Oxford OX1 3RB, England Univ Oxford Oxford England OX1 3RB pt Plant Sci, Oxford OX1 3RB, England Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England Univ Warwick Coventry W Midlands England CV4 7AL 7AL, W Midlands, England
Titolo Testata:
PLANT CELL
fascicolo: 5, volume: 11, anno: 1999,
pagine: 849 - 864
SICI:
1040-4651(199905)11:5<849:BSDANP>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASTOME-ENCODED GENES; LARGE SUBUNIT GENE; HORDEUM-VULGARE-L; CELLULAR-DIFFERENTIATION; MOLECULAR CHAPERONE; ESCHERICHIA-COLI; LEAF DEVELOPMENT; PROTOCHLOROPHYLLIDE OXIDOREDUCTASE; CHLOROPLAST DEVELOPMENT; MUTANT PHENOTYPE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
66
Recensione:
Indirizzi per estratti:
Indirizzo: Langdale, JA Univ Oxford, Dept Plant Sci, S Parks Rd, Oxford OX1 3RB, England Univ Oxford S Parks Rd Oxford England OX1 3RB 1 3RB, England
Citazione:
T.P. Brutnell et al., "BUNDLE SHEATH DEFECTIVE2, a novel protein required for post-translational regulation of the rbcL gene of maize", PL CELL, 11(5), 1999, pp. 849-864

Abstract

The Bundle sheath defective2 (Bsd2) gene is required for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) accumulation in maize, Using a Mutator transposable element as a molecular probe, we identified a tightly linked restriction fragment length polymorphism that cosegregated with the bsd2-conferred phenotype, This fragment was cloned, and sequences flanking theMutator insertion were used to screen a maize leaf cDNA library. Using a full-length cDNA clone isolated in this screen, we show that an abundant 0.6-kb transcript could be detected in wild-type plants but not in bsd2-m1 plants. This 0.6-kb transcript accumulated to low levels in plants carrying anallele derived from bsd2-m1 that conditions a less severe mutant phenotype. Taken together, these data strongly suggest that we have cloned the Bsd2 gene. Sequence analysis of the full-length cDNA clone revealed a chloroplast targeting sequence and a region of homology shared between BSD2 and the DnaJ class of molecular chaperones. This region of homology is limited to a cysteine-rich Zn binding domain in DnaJ believed to play a role in protein-protein interactions. We show that BSD2 is targeted to the chloroplast but is not involved in general photosynthetic complex assembly or protein import. In bsd2 mutants, we could not detect the Rubisco protein, but the chloroplast-encoded Rubisco large subunit transcript (rbcL) was abundant and associated with polysomes in both bundle sheath and mesophyll cells. By characterizing Bsd2 expression patterns and analyzing the bsd2-conferred phenotype, we propose a model for BSD2 in the post-translational regulation of rbcL in maize.

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Documento generato il 03/04/20 alle ore 04:24:26