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Titolo:
Differential distribution of urocortin- and corticotropin-releasing factor-like immunoreactivities in the rat brain
Autore:
Morin, SM; Ling, N; Liu, XJ; Kahl, SD; Gehlert, DR;
Indirizzi:
Eli Lilly & Co, Lilly Res Labs, Indianapolis, IN 46285 USA Eli Lilly & CoIndianapolis IN USA 46285 Labs, Indianapolis, IN 46285 USA
Titolo Testata:
NEUROSCIENCE
fascicolo: 1, volume: 92, anno: 1999,
pagine: 281 - 291
SICI:
0306-4522(1999)92:1<281:DDOUAC>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR CRF; NEUROPEPTIDE; BINDING; SYSTEM; LOCALIZATION; HYPOTHALAMUS; INCREASE; NUCLEUS;
Keywords:
urocortin; corticotropin-releasing factor; immunohistochemistry; supraoptic nucleus; Edinger-Westphal nucleus; sphenoid nucleus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Gehlert, DR Eli Lilly & Co, Lilly Res Labs, Indianapolis, IN 46285 USA EliLilly & Co Indianapolis IN USA 46285 apolis, IN 46285 USA
Citazione:
S.M. Morin et al., "Differential distribution of urocortin- and corticotropin-releasing factor-like immunoreactivities in the rat brain", NEUROSCIENC, 92(1), 1999, pp. 281-291

Abstract

Urocortin, a novel 40 amino acid neuropeptide, is a member of the corticotropin-releasing factor family. With 45% homology to corticotropin-releasingfactor, urocortin binds with similar affinity to the corticotropin-releasing factor-1 and corticotropin-releasing factor-2 receptors and may play a role in modulating many of the same systems as corticotropin-releasing factor. To assess whether urocortin and corticotropin-releasing factor are localized in the same regions of the brain, we compared the distribution of urocortin- and corticotropin-releasing factor-like immunoreactivities in the rat central nervous system. Polyclonal antibodies to rat corticotropin-releasing factor and rat urocortin were generated and utilized to map the distribution of corticotropin-releasing factor- and urocortin-like immunoreactivities throughout the rat forebrain and brainstem. Characterization of the antibodies by radioimmunoassay showed no crossreactivity with related peptides. Male Sprague-Dawley rats were treated with colchicine for 18-24 h. Following colchicine treatment, the rats were perfused with paraformaldehyde-lysine-periodate fixative and their brains removed. Serial coronal sections were taken throughout the rat brain and processed for either corticotropin-releasing factor- or urocortin-like immunoreactivity. Urocortin-like immunoreactivity shows a discrete localization within several regions including the supraoptic nucleus, the median eminence, Edinger-Westphal nucleus and the sphenoid nucleus. This is in contrast to the more abundant corticotropin-releasing factor-like immunoreactivity. Regions containing high levels of corticotropin-releasing factor immunoreactivity include the lateral septum, paraventricular nucleus of the hypothalamus, median eminence and locus coeruleus. There are a few regions that contain both urocortin-immunoreactive and corticotropin-releasing factor-immunoreactive cells, such as the supraoptic nucleus and the hippocampus. Therefore, urocortin and corticotropin-releasingfactor appear to have different distribution patterns which may be indicative of their respective physiological functions. (C) 1999 IBRO. Published by Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/08/20 alle ore 23:11:34