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Titolo:
An explanation for observed estrogen receptor binding to single-stranded estrogen-responsive element DNA
Autore:
Driscoll, MD; Sathya, G; Saidi, LF; DeMott, MS; Hilf, R; Bambara, RA;
Indirizzi:
Univ Rochester, Sch Med & Dent, Dept Biochem & Biophys, Rochester, NY 14642 Univ Rochester Rochester NY USA 14642 chem & Biophys, Rochester, NY 14642
Titolo Testata:
MOLECULAR ENDOCRINOLOGY
fascicolo: 6, volume: 13, anno: 1999,
pagine: 958 - 968
SICI:
0888-8809(199906)13:6<958:AEFOER>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
EUKARYOTIC TRANSCRIPTIONAL ACTIVATORS; WORK;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Bambara, RA Univchester,er, Sch Med & Dent, Dept Biochem & Biophys, 575 Elmwood Ave, Ro Univ Rochester 575 Elmwood Ave Rochester NY USA 14642 Ave, Ro
Citazione:
M.D. Driscoll et al., "An explanation for observed estrogen receptor binding to single-stranded estrogen-responsive element DNA", MOL ENDOCR, 13(6), 1999, pp. 958-968

Abstract

Estrogen-inducible genes contain an enhancer called the estrogen response element (ERE), a double-stranded inverted repeat. The estrogen receptor (ER) is generally thought to bind to the double-stranded ERE. However, some reports provide evidence that an ER homodimer can bind a single strand of theERE and suggest that single-stranded ERE binding is the preferred binding mode for ER. Since these two models describe quite different mechanisms of receptor action, we have attempted to reconcile the observations. AnalyzingDNA structure by nuclease sensitivity, we found that two identical molecules of a single strand of DNA containing the ERE sequence can partially anneal in an antiparallel manner. Bimolecular annealing produces double-stranded inverted repeats, with adjacent unannealed tails. The amount of annealingcorrelates exactly with the ability of ER to bind bimolecular EREs. Eitherstrand of an ERE could anneal to itself in a way that would bind ER. We conclude that ER binds only the annealed double-stranded ERE both in vitro and in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 21:41:10