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Titolo:
Promoter activity of mouse kappa opioid receptor gene in transgenic mouse
Autore:
Hu, XL; Cao, S; Loh, HH; Wei, LN;
Indirizzi:
Univ Minnesota, Sch Med, Dept Pharmacol, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 rmacol, Minneapolis, MN 55455 USA
Titolo Testata:
MOLECULAR BRAIN RESEARCH
fascicolo: 1, volume: 69, anno: 1999,
pagine: 35 - 43
SICI:
0169-328X(19990521)69:1<35:PAOMKO>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
MORPHINE-INDUCED ANALGESIA; MESSENGER-RNA; CELL-LINE; MICE; EXPRESSION; BRAIN; LIGAND; SENSITIVITY; WITHDRAWAL; PEPTIDES;
Keywords:
kappa opioid receptor; transgenic mouse; gene expression; promoter; embryo;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Wei, LN Univ Minnesota, Sch Med, Dept Pharmacol, 3-249 Millard Hall,435 Delaware St Univ Minnesota 3-249 Millard Hall,435 Delaware St SE Minneapolis MN USA 55455
Citazione:
X.L. Hu et al., "Promoter activity of mouse kappa opioid receptor gene in transgenic mouse", MOL BRAIN R, 69(1), 1999, pp. 35-43

Abstract

The biological activity of mouse kappa opioid receptor (KOR) gene promoterwas examined in transgenic mice using a beta-galactosidase (lacZ) reporterstrategy for the first time. A lacZ cDNA was inserted at the 5th amino acid in the coding region of a mouse KOR genomic segment containing 3 kb of the 5' regulatory region, to generate a Kor-lacZ fusion gene which was then used to generate transgenic mice. The expression of transgene was demonstrated at the RNA level by reverse transcription-polymerase chain reaction (RT-PCR), and at the protein level by in situ lacZ enzyme assay. From studying three independent transgenic mouse lines that express this transgene, it isconcluded that Kor-lacZ expression begins at embryonic day 9.5 (E9.5) and increases in several brain areas and neural tube as embryos develop. At E12.5 and E13.5, Kor-lacZ expression is found primarily in the mantle layer ofmidbrain, hindbrain and medulla oblongata, cranial ganglion and vagus nerve. At E15.5 and E17.5, the transgene is expressed in eye, ear, neopallial cortex, caudate putamen, lateral ventricle, thalamus, hypothalamus and pens. Therefore, the 3 kb 5' regulatory sequence of the mouse KOR gene is functional in transgenic animals and directs a specific expression pattern recapitulating that of the endogenous KOR gene expression during developmental stages. However, in adult animals, this transgene is only expressed in the brain, indicating that the regulatory information for peripheral expression in the adult is not encoded within this 3 kb upstream sequence. (C) 1999 Elsevier Science B.V. All rights reserved.

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Documento generato il 13/07/20 alle ore 18:09:54