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Titolo:
Anti-proliferative capsular-like polysaccharide antigen from Actinobacillus actinomycetemcomitans induces apoptotic cell death in mouse osteoblastic MC3T3-E1 cells
Autore:
Yamamoto, S; Mogi, M; Kinpara, K; Ishihara, Y; Ueda, N; Amano, K; Nishihara, T; Noguchi, T; Togari, A;
Indirizzi:
Aichi Gakuin Univ, Sch Dent, Dept Pharmacol, Nagoya, Aichi 4648650, Japan Aichi Gakuin Univ Nagoya Aichi Japan 4648650 Nagoya, Aichi 4648650, Japan Natl Inst Infect Dis, Dept Oral Sci, Tokyo, Japan Natl Inst Infect Dis Tokyo Japan nfect Dis, Dept Oral Sci, Tokyo, Japan Aichi Gakuin Univ, Sch Dent, Dept Periodontol, Nagoya, Aichi 4648650, Japan Aichi Gakuin Univ Nagoya Aichi Japan 4648650 Nagoya, Aichi 4648650, Japan
Titolo Testata:
JOURNAL OF DENTAL RESEARCH
fascicolo: 6, volume: 78, anno: 1999,
pagine: 1230 - 1237
SICI:
0022-0345(199906)78:6<1230:ACPAFA>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN PERIODONTAL-DISEASE; NITRIC-OXIDE; TNF-ALPHA; BONE-RESORPTION; INHIBITION; MACROPHAGES; EXPRESSION; BCL-2; FAS; Y4;
Keywords:
A-actinomycetemcomitans; apoptosis; DNA fragmentation; MC3T3-E1; SaOS-(2);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Mogi, M Aichi Gakuin Univ, Sch Dent, Dept Pharmacol, Nagoya, Aichi 4648650, Japan Aichi Gakuin Univ Nagoya Aichi Japan 4648650 Aichi 4648650, Japan
Citazione:
S. Yamamoto et al., "Anti-proliferative capsular-like polysaccharide antigen from Actinobacillus actinomycetemcomitans induces apoptotic cell death in mouse osteoblastic MC3T3-E1 cells", J DENT RES, 78(6), 1999, pp. 1230-1237

Abstract

Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans) has been implicated in the etiology of localized juvenile periodontitis (LJP), and produces a multiplicity of tissue-damaging products. Among those products, the capsular-like polysaccharide antigen (CPA) from A. actinomycetemcomitans is a potent mediator of bone resorption. In fact, this CPA (serotype b) is known to promote osteoclast-like cell formation via interleukin (IL)-1 alpha production in mouse marrow cultures. Although osteoblasts complete bone formation, there are few reports focusing on the effect of CPA in bone-forming activity of osteoblasts in inflammatory disease sites. We hypothesized that CPA plays a mediating role in osteoblastic cells. Therefore, the purpose of this study was to examine the effect of CPA from A. actinomycetemcomitans ns on the mouse osteoblastic cell line MC3T3-E1 and human osteosarcoma SaOS-2 cells. A. actinomycetemcomitans serotype c resulted in a potent dose-dependent inhibition of cell proliferation of both cell lines. Characterization of the antiproliferative activity in the CPA demonstrated that it wasnot cytotoxic for MC3T3-E1. A 20-hour incubation with CPA-c resulted in a significant increase in apoptotic cell death in the cells, as evaluated by both cellular DNA fragmentation ELISA and FAGS analysis. In contrast to theresults obtained with a cytokine mixture (tumor necrosis factor-alpha, IL-1 beta, and interferon-gamma), no inducible nitric oxide (NO) synthase geneexpression or NO release could be detected in MC3T3-E1 after incubation with CPA-c. Further, both CPA-b and -c caused potent induction of ayoptosis-related modifiers, e.g., Fas mRNA, whereas bcl-2 mRNA levels were unchanged. Therefore, this study has shown that CPA from A. actinomycetemcomitans contains a potent antiproliferative polysaccharide whose activity is associated with apoptotic cell death in MC3T3-E1, and that CPA per se is an inducer of apoptosis mediated by the Fas system but not by NO.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 11:04:06