Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
"Action-at-a-distance" mutagenesis - 8-oxo-7,8-dihydro-2 '-deoxyguanosine causes base substitution errors at neighboring template sites when copied by DNA polymerase beta
Autore:
Efrati, E; Tocco, G; Eritja, R; Wilson, SH; Goodman, MF;
Indirizzi:
Univ So Calif, Dept Biol Sci, Los Angeles, CA 90089 USA Univ So Calif LosAngeles CA USA 90089 iol Sci, Los Angeles, CA 90089 USA Univ So Calif, Dept Chem, Hedco Mol Biol Labs, Los Angeles, CA 90089 USA Univ So Calif Los Angeles CA USA 90089 ol Labs, Los Angeles, CA 90089 USA European Mol Biol Org, D-69012 Heidelberg, Germany European Mol Biol Org Heidelberg Germany D-69012 012 Heidelberg, Germany NIEHS, Res Triangle Pk, NC 27709 USA NIEHS Res Triangle Pk NC USA 27709NIEHS, Res Triangle Pk, NC 27709 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 22, volume: 274, anno: 1999,
pagine: 15920 - 15926
SICI:
0021-9258(19990528)274:22<15920:"M-8'C>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI; 8-HYDROXYGUANINE 7,8-DIHYDRO-8-OXOGUANINE; NUCLEOSIDE TRIPHOSPHATASE; MUTT PROTEIN; REPLICATION; INSERTION; FIDELITY; EXCISION; LESION; REPAIR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Goodman, MF Univ So Calif, Dept Biol Sci, Los Angeles, CA 90089 USA Univ So Calif Los Angeles CA USA 90089 Angeles, CA 90089 USA
Citazione:
E. Efrati et al., ""Action-at-a-distance" mutagenesis - 8-oxo-7,8-dihydro-2 '-deoxyguanosine causes base substitution errors at neighboring template sites when copied by DNA polymerase beta", J BIOL CHEM, 274(22), 1999, pp. 15920-15926

Abstract

8-Oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dG), a common oxidative DNA lesion, favors a syn-conformation in DNA, enabling formation of stable 8-oxo-dG A base mispairs resulting in G.C --> T.A transversion mutations. When human DNA polymerase (pol) beta was used to copy a short single-stranded gap containing a site-directed 8-oxo-dG lesion, incorporation of dAMP opposite 8-oxo-dG was slightly favored over dCMP depending on "downstream" sequence context. Unexpectedly, however, a significant increase in dCMP A and dGMP A mispairs was also observed at the "upstream" 3'-template site adjacent to the lesion. Errors at these undamaged template sites occurred in four sequence contexts with both gapped and primed single-stranded DNA templates, but not when pol alpha replaced pol beta. Error rates at sites adjacent to 8-oxo-dG were roughly 1% of the values opposite 8-oxo-dG:, potentially generating tandem mutations during in vivo short-gap repair synthesis by pol beta, When 8-oxo-dG was replaced with 8-bromo-2'-deoxyguanosine, incorporation ofdCMP was strongly favored by both enzymes, with no detectable misincorporation occurring at neighboring template sites.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 22:43:21