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Titolo:
Cloning and characterization of the genes encoding a cytochrome P450 (PipA) involved in piperidine and pyrrolidine utilization and its regulatory protein (PipR) in Mycobacterium smegmatis mc(2)155
Autore:
Poupin, P; Ducrocq, V; Hallier-Soulier, S; Truffaut, N;
Indirizzi:
Univ Technol Compiegne, Lab Genet Microbienne, Ctr Rech, F-60205 Compiegne, Univ Technol Compiegne Compiegne France F-60205 Rech, F-60205 Compiegne,
Titolo Testata:
JOURNAL OF BACTERIOLOGY
fascicolo: 11, volume: 181, anno: 1999,
pagine: 3419 - 3426
SICI:
0021-9193(199906)181:11<3419:CACOTG>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPLETE GENOME SEQUENCE; ESCHERICHIA-COLI; NUCLEOTIDE-SEQUENCE; SUICIDE VECTORS; DEGRADATION; EXPRESSION; MORPHOLINE; PYRENE; STRAIN; TRANSFORMATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Truffaut, N UnivCompiegne,ompiegne, Lab Genet Microbienne, Ctr Rech, BP 20529, F-60205 Univ Technol Compiegne BP 20529 Compiegne France F-60205 0205
Citazione:
P. Poupin et al., "Cloning and characterization of the genes encoding a cytochrome P450 (PipA) involved in piperidine and pyrrolidine utilization and its regulatory protein (PipR) in Mycobacterium smegmatis mc(2)155", J BACT, 181(11), 1999, pp. 3419-3426

Abstract

Transposon mutagenesis of Mycobacterium smegmatis mc(2)155 enabled the isolation of a mutant strain (called LGM1) altered in the regulation of piperidine and pyrrolidine utilization. The complete nucleotide sequence of the gene inactivated in mutant LGM1 was determined from the wild-type strain. This gene (pipR) encoded a member of the GntR family of bacterial regulatory proteins. An insertion element (IS1096), previously described for M. smegmatis, was detected downstream of the gene pipR. Three additional open reading frames were found downstream of IS1096. The first open reading frame (pipA) appeared to encode a protein identified as a cytochrome P450 enzyme. This gene is the first member of a new family, CYP151. By a gene replacement experiment, it was demonstrated that the cytochrome P450 pipA gene is required for piperidine and pyrrolidine utilization in M. smegmatis mc2155. Geneshomologous to pipA were detected by hybridization in several, previously isolated, morpholine-degrading mycobacterial strains. A gene encoding a putative [3Fe-4S] ferredoxin (orf1) and a truncated gene encoding a putative glutamine synthetase (orf2') were found downstream of pipA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 12:43:55