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Titolo:
Cytochemical and Western blot analysis of the subcompartmentalization of the acrosome in rodents using soybean lectin
Autore:
Martinez-Menarguez, JA; Abascal, I; Aviles, M; Castells, MT; Ballesta, J;
Indirizzi:
Univrcia,ia, Sch Med, Dept Cell Biol, Sect Histol & Gen Embryol, E-30071 Mu Univ Murcia Murcia Spain E-30071 , Sect Histol & Gen Embryol, E-30071 Mu
Titolo Testata:
HISTOCHEMICAL JOURNAL
fascicolo: 1, volume: 31, anno: 1999,
pagine: 29 - 37
SICI:
0018-2214(199901)31:1<29:CAWBAO>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
GUINEA-PIG; MAMMALIAN FERTILIZATION; SPERMATOGENESIS; GLYCOSYLATION; LOCALIZATION; BIOGENESIS; PROTEINS; ENZYMES; ANTIGEN; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Ballesta, J Univrcia,ia, Sch Med, Dept Cell Biol, Sect Histol & Gen Embryol, E-30071 Mu Univ Murcia Murcia Spain E-30071 l & Gen Embryol, E-30071 Mu
Citazione:
J.A. Martinez-Menarguez et al., "Cytochemical and Western blot analysis of the subcompartmentalization of the acrosome in rodents using soybean lectin", HISTOCHEM J, 31(1), 1999, pp. 29-37

Abstract

In the present study, the formation and development of the acrosome duringspermiogenesis in four different rodent species (rat, mouse, hamster and guinea pig) was compared by means of cytochemical and blotting techniques using a lectin from soybean (SBA). This lectin recognizes specifically the acrosome of the four species at all steps of formation. At the ultrastructural level, SBA-binding pattern was similar in the acrosome of the rat, mouse and hamster. SBA preferentially labelled the electron-lucent area of the acrosome in early spermatids (Golgi and cap phases) and the outer region of the acrosome in mature spermatids (acrosome and maturation phase). The lectin binding pattern was more complex in the guinea pig acrosome. Three different subdomains can be established in the early acrosome of the guinea pig. The lectin bound the three subdomains but mainly a thin fold which spreads over the nucleus during the cap phase. In the acrosome phase, SBA strongly reacted with the principal segment. In contrast, no reactivity was observedin most of this segment in maturation phase spermatids. In this phase, SBAbound preferentially a thin area covering the dorsal region of the apical segment. Lectin blots of detergent-extracted testes indicated that SBA onlyrecognizes proteins of high molecular weight (> 100 kD) in the four species studied. The results obtained in the present study suggest that the development of acrosomal subdomains is very similar in the mouse, rat and hamster but shows a more complex pattern in the guinea pig.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 13:09:13