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Titolo:
Comparison of three different commercial methods for measuring plasma viraemia in patients infected with non-B HIV-1 subtypes
Autore:
Holguin, A; de Mendoza, C; Soriano, V;
Indirizzi:
Inst Salud Carlos III, Hosp Carlos 3, Infect Dis Serv, Madrid, Spain Inst Salud Carlos III Madrid Spain os 3, Infect Dis Serv, Madrid, Spain
Titolo Testata:
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
fascicolo: 4, volume: 18, anno: 1999,
pagine: 256 - 259
SICI:
0934-9723(199904)18:4<256:COTDCM>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNODEFICIENCY-VIRUS TYPE-1; POLYMERASE CHAIN-REACTION; BRANCHED-DNA ASSAY; ANTIRETROVIRAL THERAPY; RNA; QUANTIFICATION; QUANTITATION; ZIDOVUDINE; PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Soriano, V C Rafael Calvo 7,2A, E-28010 Madrid, Spain C Rafael Calvo 7,2AMadrid Spain E-28010 -28010 Madrid, Spain
Citazione:
A. Holguin et al., "Comparison of three different commercial methods for measuring plasma viraemia in patients infected with non-B HIV-1 subtypes", EUR J CL M, 18(4), 1999, pp. 256-259

Abstract

In order to determine whether commercial assays presently in use for the quantification of plasma human immunodeficiency virus type 1 (HIV-1) RNA levels detect different genetic viral subtypes with the same reliability, a panel of 38 samples corresponding to 22 HIV-1-infected patients, representingnon-B subtypes A-F, was examined. One to three plasma samples belonging toeach individual were tested by the second-generation HIV-1 branched DNA (bDNA) assay (Chiron, Spain), the Nuclisens assay (Organon-Teknika, Spain), the Amplicor Monitor reverse transcriptase polymerase chain reaction assay (Roche, Spain), and the Ultradirect Monitor (Roche) using primers specifically designed to amplify non-B HIV-1 subtypes. Each of the different methods for measuring viral load showed a distinct sensitivity for non-B HIV-1 subtypes. Values higher than the assay detection limit were obtained in 22 (57.9%), 33 (86.8%), and 37 (93.3%) samples using the bDNA, Nuclisens, and Monitor assays, respectively. Significantly different values (>0.5 logs) were found in 55.3%, 81.6%, and 71.1% of specimens comparing results provided by bDNA and Nuclisens, bDNA and Monitor, and Nuclisens and Monitor, respectively. Quantitative values provided by the Ultradirect Monitor test using non-B primers were particularly discordant with the other tests. Overall, 44.7%of samples yielded higher viral load values with this assay than with the regular Monitor assay, reflecting its enhanced sensitivity for non-B subtypes; however, the reproducibility of this test was low. These results support the recommendation of always using the same assay when monitoring plasma viraemia.

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Documento generato il 02/04/20 alle ore 02:57:16