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Titolo:
Inefficient processing impairs release of RNA from the site of transcription
Autore:
Custodio, N; Carmo-Fonseca, M; Geraghty, F; Pereira, HS; Grosveld, F; Antoniou, M;
Indirizzi:
Guys Hosp, GKT Med Sch, Dept Expt Pathol, London SE1 9RT, England Guys Hosp London England SE1 9RT pt Expt Pathol, London SE1 9RT, England Univ Lisbon, Inst Histol & Embryol, Fac Med, P-1699 Lisbon, Portugal Univ Lisbon Lisbon Portugal P-1699 yol, Fac Med, P-1699 Lisbon, Portugal Erasmus Univ, MGC Dept Cell Biol & Genet, NL-3015 GE Rotterdam, Netherlands Erasmus Univ Rotterdam Netherlands NL-3015 GE GE Rotterdam, Netherlands
Titolo Testata:
EMBO JOURNAL
fascicolo: 10, volume: 18, anno: 1999,
pagine: 2855 - 2866
SICI:
0261-4189(19990517)18:10<2855:IPIROR>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRE-MESSENGER-RNA; DOMINANT CONTROL REGION; BETA-GLOBIN LOCUS; II LARGEST SUBUNIT; C-TERMINAL DOMAIN; POLYMERASE-II; NUCLEAR MATRIX; IN-VIVO; SPLICING FACTORS; ALPHA-AMANITIN;
Keywords:
pre-mRNA processing; RNA transport; transcription;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
64
Recensione:
Indirizzi per estratti:
Indirizzo: Antoniou, M Guys Hosp, GKT Med Sch, Dept Expt Pathol, London SE1 9RT, England Guys Hosp London England SE1 9RT ol, London SE1 9RT, England
Citazione:
N. Custodio et al., "Inefficient processing impairs release of RNA from the site of transcription", EMBO J, 18(10), 1999, pp. 2855-2866

Abstract

We describe here for the first time the site of retention within the nucleus of pre-mRNA processing mutants unable to be exported to the cytoplasm, Fluorescence in situ hybridization was used to detect transcripts from humanbeta-globin genes that are either normal or defective in splicing or 3' end formation. Nuclear transcripts of both wild-type and mutant RNAs are detected only as intranuclear foci that colocalize with the template gene locus. The kinetics of transcript release from the site of transcription was assessed by treatment of cells with the transcriptional inhibitors actinomycinD, alpha-amanitin and DRB, These drugs induce the rapid disappearance of nuclear foci corresponding to wild-type human beta-globin RNA. In contrast, pre-mRNA mutants defective in either splicing or 3' end formation and whichfail to be transported to the cytoplasm, are retained at the site of transcription. Therefore, 3' end processing and splicing appear to be rate limiting for release of mRNA from the site of transcription.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 15:18:49