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Titolo:
Early expression of ubiquitin in myofibers of rats in organophosphate intoxication
Autore:
Calore, EE; Sesso, A; Puga, FR; Cavaliere, MJ; Calore, NMP; Weg, R;
Indirizzi:
Emilio Ribas Inst, Pathol Sect, BR-01246902 Sao Paulo, Brazil Emilio RibasInst Sao Paulo Brazil BR-01246902 BC46902 Sao Paulo, Brazil Univ Sao Paulo, Sch Med, Dept Pathol, Lab Mol Pathol, Sao Paulo, Brazil Univ Sao Paulo Sao Paulo Brazil thol, Lab Mol Pathol, Sao Paulo, Brazil Inst Biol, Toxicol Sect, Sao Paulo, Brazil Inst Biol Sao Paulo BrazilInst Biol, Toxicol Sect, Sao Paulo, Brazil Adolfo Lutz Inst, Div Pathol, Sao Paulo, Brazil Adolfo Lutz Inst Sao Paulo Brazil z Inst, Div Pathol, Sao Paulo, Brazil
Titolo Testata:
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
fascicolo: 2, volume: 43, anno: 1999,
pagine: 187 - 194
SICI:
0147-6513(199906)43:2<187:EEOUIM>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
SKELETAL-MUSCLE; PROTEOLYTIC PATHWAY; MUSCULAR-DYSTROPHY; MYOPATHY; DEGRADATION; PROTEINS; LYSOSOMES;
Keywords:
toxic myopathies; ubiquitin; necrosis; organophosphates; proteolytic pathways; lysosome;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Calore, EE Emiliorazils Inst, Pathol Sect, Av Dr Arnaldo 165, BR-01246902 Sao Paulo, B Emilio Ribas Inst Av Dr Arnaldo 165 Sao Paulo Brazil BR-01246902 BC
Citazione:
E.E. Calore et al., "Early expression of ubiquitin in myofibers of rats in organophosphate intoxication", ECOTOX ENV, 43(2), 1999, pp. 187-194

Abstract

The degenerative process of the myofibers of the diaphragm of rats intoxicated with the organophosphate isofenphos, a compound that inhibits esterases, was studied at different intervals of intoxication. Early disorganization of the intermyofibrillar network and of the myofilaments, as well as dilatation of organelles, were observed by use of transmission electron microscopy, These changes precede macrophage invasion of the muscle fibers. Early expression of ubiquitin was observed in segments of muscle fibers by immunohistochemistry. Bands of polyubiquitin complexes in muscle homogenates wereobserved by immunoblotting, These bands disappeared in later stages of intoxication. A 42.5-kDa band corresponds to actin, as observed by immunoblotting using antisarcometric actin, This indicates relatively large amounts ofpolyubiquitin complex associated with sarcomeric actin in muscle fibers inearly stages of intoxication. Based on these results it seems that actin is an important target in organophosphate-induced myofiber degradation and that the degradation of this protein-by the polyubiquitin pathway-may play an important role in the early disorganization of the sarcomere, as observedby electron microscopy, A possible role of the ubiquitin proteolytic pathway is that of trying to eliminate proteins modified in the early phases of muscle fiber degeneration, which is a necessary step for regeneration of the posterior segmental muscle. (C) 1999 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 17/02/20 alle ore 08:12:24