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Titolo:
Ectopic expression of individual E(spl) genes has differential effects on different cell fate decisions and underscores the biphasic requirement for Notch activity in wing margin establishment in Drosophila
Autore:
Ligoxygakis, P; Bray, SJ; Apidianakis, Y; Delidakis, C;
Indirizzi:
Fdneece & Technol Hellas, Inst Mol Biol & Biotechnol, GR-71110 Iraklion, Gr Fdn Res & Technol Hellas Iraklion Greece GR-71110 GR-71110 Iraklion, Gr Univ Crete, Dept Biol, GR-71110 Iraklion, Greece Univ Crete Iraklion Greece GR-71110 Dept Biol, GR-71110 Iraklion, Greece Univ Cambridge, Dept Anat, Cambridge CB2 1TN, England Univ Cambridge Cambridge England CB2 1TN nat, Cambridge CB2 1TN, England
Titolo Testata:
DEVELOPMENT
fascicolo: 10, volume: 126, anno: 1999,
pagine: 2205 - 2214
SICI:
0950-1991(199905)126:10<2205:EEOIEG>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACHAETE-SCUTE EXPRESSION; SPLIT COMPLEX GENES; SIGNALING PATHWAY; NEURAL DEVELOPMENT; PATTERN-FORMATION; ENHANCER; LOCUS; DELTA; CUT; NEUROGENESIS;
Keywords:
Drosophila; E(spl); wing development; bristle development; wing veins; Notch signalling;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Delidakis, C Fdn1110 & Technol Hellas, Inst Mol Biol & Biotechnol, Vasilika Vouton, GR-7 Fdn Res & Technol Hellas Vasilika Vouton Iraklion Greece GR-71110
Citazione:
P. Ligoxygakis et al., "Ectopic expression of individual E(spl) genes has differential effects on different cell fate decisions and underscores the biphasic requirement for Notch activity in wing margin establishment in Drosophila", DEVELOPMENT, 126(10), 1999, pp. 2205-2214

Abstract

A common consequence of Notch signalling in Drosophila is the transcriptional activation of seven Enhancer of split [E(spl)] genes, which encode a family of closely related basic-helix-loop-helix transcriptional repressors. Different E(spl) proteins can functionally substitute for each other, hampering loss-of-function genetic analysis and raising the question of whether any specialization exists within the family. We expressed each individual E(spl) gene using the GAL4-UAS system in order to analyse their effect in a number of cell fate decisions taking place in the wing imaginal disk. We focussed on sensory organ precursor determination, wing vein determination and wing pattern formation, All of the E(spl) proteins affect the first two processes in the same way, namely they antagonize neural precursor and vein fates. Yet, the efficacy of this antagonism is quite distinct: E(spl)mp hasthe strongest vein suppression effect, whereas E(spl)m8 and E(spl)m7 are the most active bristle suppressors. During wing patterning, Notch activity orchestrates a complex sequence of events that define the dorsoventral boundary of the wing. We have discerned two phases within this process based onthe sensitivity of N loss-of-function phenotypes to concomitant expressionof E(spl) genes. E(spl) proteins are initially involved in repression of the vg quadrant enhancer, whereas later they appear to relay the Notch signal that triggers activation of cut expression. Of the seven proteins, E(spl)my is most active in both of these processes. In conclusion, E(spl) proteins have partially redundant functions, yet they have evolved distinct preferences in implementing different cell fate decisions, which closely match their individual normal expression patterns.

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Documento generato il 25/11/20 alle ore 14:57:42