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Titolo:
A role for serum response factor in coronary smooth muscle differentiationfrom proepicardial cells
Autore:
Landerholm, TE; Dong, XR; Lu, J; Belaguli, NS; Schwartz, RJ; Majesky, MW;
Indirizzi:
Baylor Coll Med, Dept Pathol, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 , Dept Pathol, Houston, TX 77030 USA Baylor Coll Med, Dept Cell Biol, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 ept Cell Biol, Houston, TX 77030 USA Baylor Coll Med, Grad Program Cardiovasc Sci, Houston, TX 77030 USA BaylorColl Med Houston TX USA 77030 ardiovasc Sci, Houston, TX 77030 USA
Titolo Testata:
DEVELOPMENT
fascicolo: 10, volume: 126, anno: 1999,
pagine: 2053 - 2062
SICI:
0950-1991(199905)126:10<2053:ARFSRF>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIN GENE-TRANSCRIPTION; HEAVY-CHAIN GENE; EXTRACELLULAR-MATRIX; CHICK-EMBRYO; FACTOR SRF; EXPRESSION; PROMOTER; ORIGIN; ACTIVATION; ELEMENTS;
Keywords:
epicardium; coronary artery; calponin; cardiac development;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Majesky, MW Baylor Coll Med, Dept Pathol, 1 Baylor Plaza, Houston, TX 77030 USA Baylor Coll Med 1 Baylor Plaza Houston TX USA 77030 77030 USA
Citazione:
T.E. Landerholm et al., "A role for serum response factor in coronary smooth muscle differentiationfrom proepicardial cells", DEVELOPMENT, 126(10), 1999, pp. 2053-2062

Abstract

Coronary artery smooth muscle (SM) cells originate from proepicardial cells that migrate over the surface of the heart, undergo epithelial to mesenchymal transformation and invade the subepicardial and cardiac matrix. Prior to contact with the heart, proepicardial cells exhibit no expression of smooth muscle markers including SM alpha actin, SM22 alpha, calponin, SM gammaactin or SM-myosin heavy chain detectable by RT-PCR or by immunostaining. To identify factors required for coronary smooth muscle differentiation, weexcised proepicardial cells from Hamburger-Hamilton stage-17 quail embryosand examined them ex vivo. Proepicardial cells initially formed an epithelial colony that was uniformly positive for cytokeratin, an epicardial marker. Transcripts for flk-1, Nkx 2.5, GATA4 or smooth muscle markers were undetectable, indicating an absence of endothelial, myocardial or preformed smooth muscle cells. By 24 hours, cytokeratin-positive cells became SM alpha actin-positive. Moreover, serum response factor, undetectable in freshly isolated proepicardial cells, became strongly expressed in virtually all epicardial cells. By 72 hours, a subset of epicardial cells exhibited a rearrangement of cytoskeletal actin, focal adhesion formation and acquisition of a motile phenotype. Coordinately with mesenchymal transformation, calponin, SM22a and SM gamma actin became expressed. By 5-10 days, SM-myosin heavy chain mRNA was found, by which time nearly all cells had become mesenchymal. RT-PCR showed that large increases in serum response factor expression coincide with smooth muscle differentiation in vitro. Two different dominant-negative serum response factor constructs prevented the appearance of calponin-, SM22 alpha- and SM gamma actin-positive cells. By contrast, dominant-negative serum response factor did not block mesenchymal transformation nor significantly reduce the number of cytokeratin-positive cells. These results indicate that the stepwise differentiation of coronary smooth muscle cells from proepicardial cells requires transcriptionally active serum response factor.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 03:34:40