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Titolo:
Expression of the elastin promoter in novel tissue sites in transgenic mouse embryos
Autore:
Lakkakorpi, J; Li, KH; Decker, S; Korkeela, E; Piddington, R; Abrams, W; Bashir, M; Uitto, J; Rosenbloom, J;
Indirizzi:
Univ Penn, Sch Dent Med, Dept Anat & Histol, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 & Histol, Philadelphia, PA 19104 USA Thomas07efferson Univ, Dept Dermatol & Cutaneous Biol, Philadelphia, PA 191 Thomas Jefferson Univ Philadelphia PA USA 19107 iol, Philadelphia, PA 191
Titolo Testata:
CONNECTIVE TISSUE RESEARCH
fascicolo: 2, volume: 40, anno: 1999,
pagine: 155 -
SICI:
0300-8207(1999)40:2<155:EOTEPI>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-EXPRESSION; MESSENGER-RNA; FIBROBLASTS; LIGAMENT; REGION; MICE;
Keywords:
elastin promoter; transgenic mice; hyaline cartilage; neuroepithelia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Rosenbloom, J UnivPAenn, Sch Dent Med, Dept Anat & Histol, 4001 Spruce St,Philadelphia, Univ Penn 4001 Spruce St Philadelphia PA USA 19104 adelphia,
Citazione:
J. Lakkakorpi et al., "Expression of the elastin promoter in novel tissue sites in transgenic mouse embryos", CONNECT TIS, 40(2), 1999, pp. 155

Abstract

We have previously shown in a transgenic mouse line, in which 5.2 kb of the elastin promoter was linked to the reporter enzyme chloramphenicol acetyltransferase (CAT), that the highest levels of expression were found in embryonic lungs and aorta, while lower levels were detected in other elastin-containing tissues. Furthermore, in general, expression of the transgene showed developmental regulation similar to that of the endogenous gene, However, the precise location of cellular expression could not be determined in this model. To overcome this limitation, we have developed a similar model, but replaced CAT with the reporter enzyme P-galactosidase, Enzyme activity was readily detected in the transgenic mouse embryos in expected regions of tissue forming elastic fibers, including the dermis and elastic cartilage. Of considerable interest, however, was the novel finding of expression in specific areas of neuroepithelium of the brain and in the perichondrium surrounding areas destined to form hyaline cartilage in endochondral bone formation. These latter areas included all the bones of the limbs, the spine andrib cage, It appeared that these segments of elastin expression demarcatedthe border between the developing cartilage and the surrounding mesenchymal tissue. Elastin promoter expression was also found in developing somites,in the mesenchymal layer of the forming cornea of the eye, in the genital tubercle and in the epithelium destined to form the olfactory epithelium. These findings indicate that the elastin promoter is activated during embryonic development in a variety of tissues, suggesting that elastin gene expression may play a role in organizing cutaneous, skeletal and neural structures.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 23:10:22