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Titolo:
Comparative flow cytometric study of clonal excess in leukaemic peripheralblood from patients suffering from chronic lymphocytic leukaemia (B-CLL) by different antibodies, staining techniques and the effects of blood storage
Autore:
Schmidtke, G; Schmucker, U; Brittinger, G; Hoffkes, HG;
Indirizzi:
Ottoeburg,uericke Univ, Dept Internal Med, Div Haematol Oncol, D-39120 Magd Otto Von Guericke Univ Magdeburg Germany D-39120 tol Oncol, D-39120 Magd Univmanyen Gesamthsch, Dept Internal Med, Div Haematol, D-4300 Essen 1, Ger Univ Essen Gesamthsch Essen Germany 1 Div Haematol, D-4300 Essen 1, Ger
Titolo Testata:
CLINICAL AND LABORATORY HAEMATOLOGY
fascicolo: 2, volume: 21, anno: 1999,
pagine: 103 - 112
SICI:
0141-9854(199904)21:2<103:CFCSOC>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
FICOLL-HYPAQUE SEPARATION; LYSIS TECHNIQUE; NORMAL ADULTS; T-CELL; TEMPERATURE; ANTICOAGULANT; ANTIGENS; SUBSETS; FRESH;
Keywords:
chronic lymphocytic leukaemia; immunophenotyping; pre-analysis; storage;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Hoffkes, HG OttorVon Guericke Univ, Dept Internal Med, Div Haematol Oncol,Leipziger St Otto Von Guericke Univ Leipziger Str 44 Magdeburg Germany D-39120
Citazione:
G. Schmidtke et al., "Comparative flow cytometric study of clonal excess in leukaemic peripheralblood from patients suffering from chronic lymphocytic leukaemia (B-CLL) by different antibodies, staining techniques and the effects of blood storage", CLIN LAB H, 21(2), 1999, pp. 103-112

Abstract

This investigation studied the effects of cell preparation methods, different antibody panels and blood storage on antigen expression of abnormal B lymphocytes from patients with B-CLL. Blood specimens collected in Heparin de novo were processed by using conventional Hypaque-Ficoll density gradientcentrifugation and whole blood lysis. These were stored for 3 days at 4 degrees C, 24 degrees C and 30 degrees C, Although clonal excess was detectedby all antibody panels, significant differences could be observed in termsof molecules of equivalent fluorochromes (MEF/MESF units), Evaluation of 'weak and strong' staining is dependent on the antibody panel used, Immunofluorescent values for CD19 and CD45 were unchanged at 4 OC and 24 degrees C but immunoglobulin staining showed best results when blood was stored at 4 degrees C. Storage at 30 OC produced unreliable results. Abnormal B lymphocytes should be analysed immediately after the specimen is obtained, If shipment is necessary they should be kept at 4 degrees C. Surface immunoglobulins are the 'antigens' most sensitive to storage alterations. Sample alterations alone are sufficient to the correct classification of NHL, especially in the case of low-grade NHL.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 10:02:43