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Titolo:
Dilated cardiomyopathy is associated with significant changes in collagen type I/III ratio
Autore:
Pauschinger, M; Knopf, D; Petschauer, S; Doerner, A; Poller, W; Schwimmbeck, PL; Kuhl, U; Schultheiss, HP;
Indirizzi:
FreeermanyBerlin, Klinikum Benjamin Franklin, Med Clin 2, D-12200 Berlin, G Free Univ Berlin Berlin Germany D-12200 n, Med Clin 2, D-12200 Berlin, G
Titolo Testata:
CIRCULATION
fascicolo: 21, volume: 99, anno: 1999,
pagine: 2750 - 2756
SICI:
0009-7322(19990601)99:21<2750:DCIAWS>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-BETA; I COLLAGEN; QUANTITATIVE ASSESSMENT; GENE-EXPRESSION; POLARIZED-LIGHT; MATRIX; PROMOTER; TISSUE; PURIFICATION; ACTIVATION;
Keywords:
cardiomyopathy; collagen; growth substances; remodeling; polymerase chain reaction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Pauschinger, M Free,Univ Berlin, Klinikum Benjamin Franklin, Med Clin 2, Hindenburgdamm 30 Free Univ Berlin Hindenburgdamm 30 Berlin Germany D-12200
Citazione:
M. Pauschinger et al., "Dilated cardiomyopathy is associated with significant changes in collagen type I/III ratio", CIRCULATION, 99(21), 1999, pp. 2750-2756

Abstract

Background-It is controversial whether myocardial fibrosis in end-stage dilated cardiomyopathy (DCM) is associated with altered collagen type I/type III (Col I/Col III) ratio. Methods and Results-Patients with DCM (ejection fraction [EF] <50%, n=12) and with mild global left ventricular dysfunction (EF >50%, n=18) were examined. Col I, Col III, and transforming growth factors-beta(1) (TGF-beta(1))and -beta(2) (TGF-beta(2))gene expression in endomyocardial biopsies was evaluated by quantitative competitive reverse transcriptase-polymerase chainreaction (qRT-PCR). Collagen content was quantified after picrosirius red and immunohistological staining and by hydroxyproline assay. In patients with EF <50%, there was a pronounced 2- to 6-fold increase of myocardial Col I mRNA abundance (P<0.01), with a corresponding 1.6-fold increase at the protein level versus that found in patients with EF >50%. The Col III mRNA abundance showed a 2.0-fold increase (P<0.04). There was a relevant shift in the Col I/Col III mRNA ratio for DCM patients (Col I/Col III, 8.2) comparedwith patients with an EF >50% (Col I/Col III, 6.4). In addition, total collagen content was increased in patients with EF <50% (n=3) (4.3 +/- 0.1%) compared with patients with EF >50% (n=8) (2.7 +/- 0.9%) (P<0.004). The biochemically determined ratio of hydroxyproline/total protein (n=12) was correlated to the Col I mRNA abundance (P<0.05, r=0.77). TGF-beta(1) and TGF-beta(2) showed elevated myocardial mRNA abundances (1- to 7-fold and 4- to 5-fold, respectively) in DCM patients. Conclusions-Differential increase of Col I and Col III leads to an increased Col I/Col III ratio in DCM myocardium. Because Col I provides substantial tensile strength and stiffness, this may contribute to systolic and in particular diastolic dysfunction in DCM.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 21:25:25