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Titolo:
Development of validated stereoselective methods for methadone determination in clinical samples
Autore:
Rudaz, S; Ortelli, D; Gex-Fabry, M; Deglon, J; Balant, L; Veuthey, JL;
Indirizzi:
Univ Geneva, Lab Pharmaceut Analyt Chem, CH-1211 Geneva 4, Switzerland Univ Geneva Geneva Switzerland 4 lyt Chem, CH-1211 Geneva 4, Switzerland Univ Geneva, Dept Psychiat, Unit Clin Res, CH-1211 Geneva, Switzerland Univ Geneva Geneva Switzerland CH-1211 Res, CH-1211 Geneva, Switzerland Phenix Fdn, Geneva, Switzerland Phenix Fdn Geneva SwitzerlandPhenix Fdn, Geneva, Switzerland
Titolo Testata:
CHIRALITY
fascicolo: 5-6, volume: 11, anno: 1999,
pagine: 487 - 494
SICI:
0899-0042(1999)11:5-6<487:DOVSMF>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOLID-PHASE EXTRACTION; PERFORMANCE LIQUID-CHROMATOGRAPHY; NITROGEN-PHOSPHORUS DETECTION; BASIC DRUGS; CAPILLARY ELECTROPHORESIS; BIOLOGICAL-FLUIDS; WHOLE-BLOOD; GAS-CHROMATOGRAPHY; PRIMARY METABOLITE; PLASMA-LEVELS;
Keywords:
6-dimethylamino-4,4-diphenyl-3-heptanone methadone; enantiomers; HPLC; clinical samples; extraction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Veuthey, JL Univzerland, Lab Pharmaceut Analyt Chem, Bd Yvoy 20, CH-1211 Geneva 4, Swit Univ Geneva Bd Yvoy 20 Geneva Switzerland 4 11 Geneva 4, Swit
Citazione:
S. Rudaz et al., "Development of validated stereoselective methods for methadone determination in clinical samples", CHIRALITY, 11(5-6), 1999, pp. 487-494

Abstract

A stereoselective analysis of methadone (Mtd) in whole blood and serum wasdeveloped using liquid chromatography on a protein based chiral stationaryphase. Liquid-liquid extraction (LLE) and solid phase extraction methods were applied before chromatographic analysis. The extraction procedure, as well as the choice of the biological matrix, showed significant differences in the extraction yield and in the precision of the assays. Serum was selected for this assay and LLE was chosen as the preparation step because of its simplicity and rapidity. The total procedure was validated and applied toclinical samples. Samples taken from 45 heroin-addicted patients were analyzed. A correlation was found between the dose administered and Mtd concentration (total and R-form), but interindividual variability of the total normalized Mtd was seen (concentration varied from 90 to 530 ng/ml). Furthermore, two populations were apparently observed with a mean Mtd concentration of 200 and 475 ng/ml, respectively. Stereoselective analyses showed that more than 50% of the patients presented a nonracemic ratio, and particularly about 25% showed a preferential metabolism of the active R-Mtd enantiomer. Therefore, the stereoselective determination of Mtd is necessary to improvethe quality of the treatment of heroin addiction. Chirality 11:487-494, 1999. (C) 1999 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 23:15:12