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Titolo:
Epithelial-mesenchymal interactions in development of the mouse fetal genital tubercle
Autore:
Kurzrock, EA; Baskin, LS; Li, YW; Cunha, GR;
Indirizzi:
Univ Calif San Francisco, Dept Urol, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Calif San Francisco, Dept Anat, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA
Titolo Testata:
CELLS TISSUES ORGANS
fascicolo: 3, volume: 164, anno: 1999,
pagine: 125 - 130
SICI:
1422-6405(1999)164:3<125:EIIDOT>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
UROGENITAL SINUS MESENCHYME; TISSUE RECOMBINANTS; UTERINE EPITHELIUM; GROWTH-FACTORS; ESTROGEN; CELLS; MICE; RAT; 5-ALPHA-REDUCTASE; DIFFERENTIATION;
Keywords:
genital tubercle; differentiation; mesenchyme; epithelium; mouse;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Baskin, LS Univ3Calif San Francisco, Dept Urol, U-575,Box 0738, San Francisco, CA 9414 Univ Calif San Francisco U-575,Box 0738 San Francisco CA USA 94143
Citazione:
E.A. Kurzrock et al., "Epithelial-mesenchymal interactions in development of the mouse fetal genital tubercle", CELLS T ORG, 164(3), 1999, pp. 125-130

Abstract

Epithelial-mesenchymal interactions play a central role in the developmentof urogenital organs. We hypothesized that normal development of the external genitalia depends upon proper mesenchymal-epithelial signaling. The mesenchyme of the adult mouse penis consists of a corpus cavernosum and proximal and distal bones. The differentiation of penile mesenchyme into bone andcartilage begins after birth and can be accelerated by androgens. After determining the sex, genital tubercles of fetal mice at gestational day 15 were removed. The genital tubercles were trypsinized and microdissected to remove the epidermis and urethra from the mesenchyme. Recombinant specimens were created by combining genital tubercle mesenchyme with genital tubercle epithelium, bladder epithelium or tail epidermis. Tissues were grafted under the renal capsule of male athymic mice. After 3 weeks of growth, grafts were removed from the kidney, weighed and stained with hematoxylin and eosin, Alcian blue and peanut agglutinin. Male and female grafts showed no difference in growth or differentiation. Intact grafts and recombinant grafts, irrespective of the epithelial source, grew significantly more than grafts of the mesenchyme only. Recombinant grafts demonstrated a significantly higher prevalence of cartilage formation and mesenchymal differentiation compared to grafts of the mesenchyme without epithelium. Since heterologous epithelium is able to induce equivalent growth and differentiation of phallic mesenchyme, epithelium carries a permissive, but critical, role in genital mesenchyme development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 22:19:35