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Titolo:
Localisation of P2X(5) and P2X(7) receptors by immunohistochemistry in ratstratified squamous epithelia
Autore:
Groschel-Stewart, U; Bardini, M; Robson, T; Burnstock, G;
Indirizzi:
Royallande & Univ Coll, Sch Med, Autonom Neurosci Inst, London NW3 2PF, Eng Royal Free & Univ Coll London England NW3 2PF Inst, London NW3 2PF, Eng Tech Univ Darmstadt, Inst Zool, D-64287 Darmstadt, Germany Tech Univ Darmstadt Darmstadt Germany D-64287 D-64287 Darmstadt, Germany
Titolo Testata:
CELL AND TISSUE RESEARCH
fascicolo: 3, volume: 296, anno: 1999,
pagine: 599 - 605
SICI:
0302-766X(199906)296:3<599:LOPAPR>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSFORMING GROWTH-FACTOR; GATED ION CHANNELS; ULTRASTRUCTURAL-LOCALIZATION; P2 RECEPTORS; CELL-GROWTH; DIFFERENTIATION; NEURONS; PURINOCEPTORS; CLONING; IMMUNOCYTOCHEMISTRY;
Keywords:
P2X(5) receptor; P2X(7) receptor; epithelia; rat (Sprague Dawley);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Burnstock, G Royalndone & Univ Coll, Sch Med, Autonom Neurosci Inst, Rowland Hill St, Lo Royal Free & Univ Coll Rowland Hill St London England NW3 2PF
Citazione:
U. Groschel-Stewart et al., "Localisation of P2X(5) and P2X(7) receptors by immunohistochemistry in ratstratified squamous epithelia", CELL TIS RE, 296(3), 1999, pp. 599-605

Abstract

In order to investigate whether purinoceptors are involved in the physiological renewal and regeneration of epithelia, we used immunohistochemical techniques on fresh frozen sections of various stratified squamous epithelialtissues (cornea, tongue, soft palate, oesophagus, vagina and footpad) of the rat and specific polyclonal antibodies to unique peptide sequences of P2X(1-7) receptors. Only two of the antibodies, anti-P2X(5) and anti-P2X(7), reacted with epithelial structures. P2X(5) immunoreactivity was mainly associated with the membranes of the proliferating and differentiating cell layers (spinous and granular layer) in both keratinised and non-keratinised epithelia and growing hair follicles. In contrast, P2X(7) immunoreactivity was clearly associated with the keratinisation process, the staining being most intense in the upper keratinised and the exfoliated layers. These findings suggest, for the first time, that P2X(5) and P2X(7) receptors play an important role in the physiological turnover of continuously regenerating cells, and further, raise the possibility that they represent novel targets for the development of pharmacological tools of potential benefit for diseases of epithelial dysfunction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 18:46:36