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Titolo:
The myeloid-lymphoid initiating cell (ML-IC) assay assesses the fate of multipotent human progenitors in vitro
Autore:
Punzel, M; Wissink, SD; Miller, JS; Moore, KA; Lemischka, IR; Verfaillie, CM;
Indirizzi:
Univ Minnesota, Dept Med, Div Hematol, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 ematol, Minneapolis, MN 55455 USA Univ Minnesota, Stem Cell Biol Program, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 rogram, Minneapolis, MN 55455 USA Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USA Princeton Univ Princeton NJ USA 08544 t Mol Biol, Princeton, NJ 08544 USA
Titolo Testata:
BLOOD
fascicolo: 11, volume: 93, anno: 1999,
pagine: 3750 - 3756
SICI:
0006-4971(19990601)93:11<3750:TMIC(A>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC STEM-CELLS; LONG-TERM CULTURE; IMMUNE-DEFICIENT MICE; HUMAN CORD-BLOOD; BONE-MARROW; LIMITING-DILUTION; STROMAL CELLS; DIFFERENTIATION; MAINTENANCE; MOUSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Verfaillie, CM Divlis,atol Oncol & Transplantat, Box 806 UMHC,420 DelawareSt SE, Minneapo Div Hematol Oncol & Transplantat Box 806 UMHC,420 DelawareSt SE Minneapolis MN USA 55455
Citazione:
M. Punzel et al., "The myeloid-lymphoid initiating cell (ML-IC) assay assesses the fate of multipotent human progenitors in vitro", BLOOD, 93(11), 1999, pp. 3750-3756

Abstract

Hematopoietic stem cells (HSC) are cells with self-renewing multilineage differentiation potential. Although engraftment in xenogeneic recipients canbe used to measure human HSC, these assays do not allow assessment of individual progenitors. We developed an in vitro assay that allows the identification of a single human bone marrow progenitor closely related to HSC, which we termed "Myeloid-Lymphoid Initiating Cell," or ML-IC, because it is capable of generating multiple secondary progenitors that can reinitiate long-term myeloid and lymphoid hematopoiesis in vitro. The assay is done in contact with murine AFT024 fetal liver stromal cells and with Flt3-Ligand, stem cell factor, and interleukin-7. In this assay, 0.2% to 1.7% of Lin (-)/34(+)/DRdim cells could generate 1 to 3 long-term culture initiating cells (LTC-IC) as well as 1 to 4 NK-IC after 4 to 6 weeks. In addition, this assay measures contribution of net-progenitor conservation and net-progenitor proliferation over time, providing insight in the fate of individual LTC-IC and NK-IC. This assay will prove useful to enumerate the number of very primitive human progenitors with multilineage differentiation potential, as wellas to evaluate future ex vivo culture conditions. (C) 1999 by The AmericanSociety of Hematology.

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Documento generato il 10/07/20 alle ore 18:44:48