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Titolo:
Competition between Li+ and Mg2+ in neuroblastoma SH-SY5Y cells: A fluorescence and P-31 NMR study
Autore:
Amari, L; Layden, B; Nikolakopoulos, J; Rong, QF; de Freitas, DM; Baltazar, G; Castro, MMCA; Geraldes, CFGC;
Indirizzi:
Loyola Univ, Dept Chem, Chicago, IL 60626 USA Loyola Univ Chicago IL USA 60626 a Univ, Dept Chem, Chicago, IL 60626 USA Univ Coimbra, Dept Biochem, Coimbra, Portugal Univ Coimbra Coimbra Portugal Coimbra, Dept Biochem, Coimbra, Portugal Univ Coimbra, Dept Zool, Coimbra, Portugal Univ Coimbra Coimbra Portugal niv Coimbra, Dept Zool, Coimbra, Portugal Univ Coimbra, Ctr Neurosci, Coimbra, Portugal Univ Coimbra Coimbra Portugal Coimbra, Ctr Neurosci, Coimbra, Portugal
Titolo Testata:
BIOPHYSICAL JOURNAL
fascicolo: 6, volume: 76, anno: 1999,
pagine: 2934 - 2942
SICI:
0006-3495(199906)76:6<2934:CBLAMI>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEAR-MAGNETIC-RESONANCE; CYTOSOLIC-FREE MAGNESIUM; MU-OPIOID RECEPTOR; INTRACELLULAR CALCIUM; AQUEOUS-SOLUTION; GEL THREADS; RAT-BRAIN; IN-VIVO; LITHIUM; TRANSPORT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: de Freitas, DM Loyola Univ, Dept Chem, 6525 N Sheridan Rd, Chicago, IL 60626 USA Loyola Univ 6525 N Sheridan Rd Chicago IL USA 60626 626 USA
Citazione:
L. Amari et al., "Competition between Li+ and Mg2+ in neuroblastoma SH-SY5Y cells: A fluorescence and P-31 NMR study", BIOPHYS J, 76(6), 1999, pp. 2934-2942

Abstract

Because Mg2+ and Li+ ions have similar chemical properties, we have hypothesized that Li+/Mg2+ competition for Mg2+ binding sites is the molecular basis for the therapeutic action of lithium in manic-depressive illness. By fluorescence spectroscopy with furaptra-loaded cells, the free intracellularMg2+ concentration within the intact neuroblastoma cells was found to increase from 0.39 +/- 0.04 mM to 0.60 +/- 0.04 mM during a 40-min Li+ incubation in which the total intracellular Li+ concentration increased from 0 to 5.5 mM. Our fluorescence microscopy observations of Li+-free and Li+-loaded cells also indicate an increase in free Mg2+ concentration upon Li+ incubation, By P-31 NMR, the free intracellular Mg2+ concentrations for Li+-free cells was 0.35 +/- 0.03 mM and 0.80 +/- 0.04 mM for Li+-loaded cells (final total intracellular Li+ concentration of 16 mM). If a Li+/Mg2+ competition mechanism is present in neuroblastoma cells, an increase in the total intracellular Li+ concentration is expected to result in an increase in the freeintracellular Mg2+ concentration, because Li+ displaces Mg2+ from its binding sites within the nerve cell. The fluorescence spectroscopy, fluorescence microscopy, and P-31 NMR spectroscopy studies presented here have shown this to be the case.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 09:52:35