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Titolo:
Probing proteomes using capillary isoelectric focusing-electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry
Autore:
Jensen, PK; Pasa-Tolic, L; Anderson, GA; Horner, JA; Lipton, MS; Bruce, JE; Smith, RD;
Indirizzi:
Pacific NW Labs, Environm Mol Sci Lab, Richland, WA 99352 USA Pacific NW Labs Richland WA USA 99352 Mol Sci Lab, Richland, WA 99352 USA
Titolo Testata:
ANALYTICAL CHEMISTRY
fascicolo: 11, volume: 71, anno: 1999,
pagine: 2076 - 2084
SICI:
0003-2700(19990601)71:11<2076:PPUCIF>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
MULTIPLY-CHARGED IONS; GENE-EXPRESSION; DNA MICROARRAY; PROTEINS; ELECTROPHORESIS; IDENTIFICATION; ACCUMULATION; SAMPLES; GENOME;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Smith, RD Pacific NW Labs, Environm Mol Sci Lab, POB 999, Richland, WA 99352 USA Pacific NW Labs POB 999 Richland WA USA 99352 land, WA 99352 USA
Citazione:
P.K. Jensen et al., "Probing proteomes using capillary isoelectric focusing-electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry", ANALYT CHEM, 71(11), 1999, pp. 2076-2084

Abstract

Unlike the genome, the proteome is exquisitely sensitive to cellular conditions and will consist of proteins having abundances dependent upon stage in the cell cycle, cell differentiation, response to environmental conditions (nutrients, temperature, stress, etc.), or disease state(s). Therefore, the study of proteomes under well-defined conditions can provide a better understanding of complex biological processes and inference of protein function. Thus, much faster, more sensitive, and precise capabilities for the characterization of cellular constituents are desired. We describe progress inthe development and initial application of the powerful combination of capillary isoelectric focusing (CIEF) and Fourier transform ion cyclotron resonance (FTICR) mass spectrometry for measurements of the proteome of the model system Escherichia coli. Isotope depletion of the growth media has been used to improve mass measurement accuracy, and the comparison of CIEF-FTICRresults for the analysis of cell lysates harvested from E. coli cultured in normal and isotopically depleted media are presented. The initial studieshave revealed 400-1000 putative proteins in the mass range 2-100 kDa from total injections of similar to 300 ng off. coli proteins in a single CIEF-FTICR analysis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 07:36:21