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Titolo:
IN-VITRO GROWTH OF MOBILIZED PERIPHERAL-BLOOD PROGENITOR CELLS IS SIGNIFICANTLY ENHANCED BY STEM-CELL FACTOR
Autore:
CESANA C; CARLOSTELLA C; MANGONI L; REGAZZI E; GARAU D; SAMMARELLI G; CARAMATTI C; ALMICI C; RIZZOLI V;
Indirizzi:
UNIV PARMA,CTR TRAPIANTI MIDOLLO OSSEO,CATTEDRA EMATOL,VIA GRAMSCI 14I-43100 PARMA ITALY UNIV PARMA,BONE MARROW TRANSPLANTAT CTR,DEPT HEMATOL I-43100 PARMA ITALY
Titolo Testata:
Stem cells
fascicolo: 3, volume: 15, anno: 1997,
pagine: 207 - 213
SICI:
1066-5099(1997)15:3<207:IGOMPP>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLONY-STIMULATING FACTOR; HEMATOPOIETIC STEM; BONE-MARROW; TRANSPLANTATION; MAFOSFAMIDE; EXPANSION; LEUKEMIA;
Keywords:
STEM CELL FACTOR; BLOOD CELL TRANSPLANTATION; LONG-TERM CULTURE INITIATING CELLS; HEMATOPOIETIC GROWTH FACTORS; G-CSF; CYCLOPHOSPHAMIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
C. Cesana et al., "IN-VITRO GROWTH OF MOBILIZED PERIPHERAL-BLOOD PROGENITOR CELLS IS SIGNIFICANTLY ENHANCED BY STEM-CELL FACTOR", Stem cells, 15(3), 1997, pp. 207-213

Abstract

The existence of primitive hematopoietic progenitors in mobilized peripheral blood is suggested by clinical, phenotypic and in vitro ceti culture evidences. In order to quantify primitive progenitors, 32 leukaphereses from 15 patients with lymphoid malignancies were investigatedfor the growth of multilineage cology-forming units (CFU-Mix), erythroid burst-forming units (BFU-E) and granulocyte-macrophage colony-forming units (CFU-GM) in the absence or presence of recombinant stem cellfactor (SCF), a cytokine which selectively controls stent cell self-renewal, proliferation and differentiation. Primitive progenitors were also quantitated by means of a long-term assay which allows the growthof cells capable of initiating and sustaining hematopoiesis in long-term culture (LTC-IC). Addition of SCE (50 ng/ml) to methyl-cellulose cultures stimulated with maximal concentrations of G-CSF, GM-CSF, interleukin 3 and erythropoietin significantly increased the growth (mean +/- SE) of CFU-Mix (7.7 +/- 1.7 versus 2.4 +/- 0.6, p less than or equal to 0.0001), BFU-E (47 +/- 10 versus 32 +/- 6, p less than or equal to 0.002) and CFU-GM (173 +/- 31 versus 112 +/- 20, p less than or equal to 0.0001). Mean (+/- SE) percentages of SCF-dependent CFU-Mix, BFU-E and CFU-GM were 60 +/- 5%, 19 +/- 5%, and 33 +/- 4%, respectively. Mean (+/- SE) LTC-IC growth per 2 x 10(6) nucleated cells was 221 +/- 53 (range, 2 to 704). Linear regression analysis demonstrated a statistically significant correlation (r = .87; p less than or equal to 0.0001) between LTC-IC and SCF-dependent progenitors. In conclusion, our data suggest that: A) the optimal quantification of mobilized progenitors requires supplementation of methylcellulose cultures with SCF, and B) in vitro detection of SCF-dependent progenitors might represent a reliable and technically simple method to assess the primitive progenitor cell content of blood cell autografts. Such in vitro evaluation of immature hematopoietic progenitors might be clinically relevant for predicting the reconstituting potential of autografts.

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Documento generato il 01/10/20 alle ore 07:11:57