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Titolo:
RAPD variation within and among natural populations of outcrossing willow-leaved foxglove (Digitalis obscura L.)
Autore:
Nebauer, SG; del Castillo-Agudo, L; Segura, J;
Indirizzi:
Univ Valencia, Fac Farm, Dept Biol Vegetal, Valencia 46100, Spain Univ Valencia Valencia Spain 46100 t Biol Vegetal, Valencia 46100, Spain Univ Valencia, Fac Farm, Dept Microbiol & Ecol, Valencia 46100, Spain UnivValencia Valencia Spain 46100 crobiol & Ecol, Valencia 46100, Spain
Titolo Testata:
THEORETICAL AND APPLIED GENETICS
fascicolo: 6-7, volume: 98, anno: 1999,
pagine: 985 - 994
SICI:
0040-5752(199905)98:6-7<985:RVWAAN>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENETIC-STRUCTURE; PLANT-POPULATIONS; AEDES-AEGYPTI; PCR MARKERS; DIVERSITY; SCROPHULARIACEAE; CONSERVATION; VARIABILITY; ALLOZYME; SIZE;
Keywords:
Digitalis obscura; AMOVA; HOMOVA; population genetics; RAPD;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Segura, J Univ100,encia, Fac Farm, Dept Biol Vegetal, Av VA Estelles S-N, Valencia 46 Univ Valencia Av VA Estelles S-N Valencia Spain 46100 lencia 46
Citazione:
S.G. Nebauer et al., "RAPD variation within and among natural populations of outcrossing willow-leaved foxglove (Digitalis obscura L.)", THEOR A GEN, 98(6-7), 1999, pp. 985-994

Abstract

Random amplified polymorphic DNA (RAPD) markers were used to assess levelsand patterns of genetic diversity in Digitalis obscura L. (Scrophulariaceae), an outcrossing cardenolide-producing medicinal plant species. A total of 50 plants from six natural populations on the Iberian Peninsula were analysed by six arbitrarily chosen decamer primers resulting in 96 highly reproducible RAPD bands. To avoid bias in parameter estimation, analyses of population genetic structure were restricted to bands (35 of 96) whose observedfrequencies were less than 1-3/n in each population. The analysis of molecular variance (AMOVA) with distances among individuals corrected for the dominant nature of RAPDs (genotypic analysis) showed that most of the variation (84.8%) occurred among individuals within populations, which is expectedfor an outcrossing organism. Of the remaining variance, 9.7% was attributed to differences between regions, and 5.5% for differences among populations within regions. Estimates of the Wright, Weir and Cockerham and Lynch andMilligan F-ST from null-allele frequencies corroborated AMOVA partitioningand provided significant evidence for population differentiation in D. obscura. A non-parametric test for the homogeneity of molecular variance (HOMOVA) also showed significant differences in the amount of genetic variability present in the six populations. UPGMA cluster analyses, based on Apostol genetic distance, revealed grouping of some geographically proximate populations. Nevertheless, a Mantel test did not give a significant correlation between geographic and genetic distances. This is the first report of the partitioning of genetic variability within and between populations of D. obscura and provides important baseline data for optimising sampling strategiesand for conserving the genetic resources of this medicinal species.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 20:56:25