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Titolo:
Evidence for specific nucleocytoplasmic transport pathways used by leucine-rich nuclear export signals
Autore:
Elfgang, C; Rosorius, O; Hofer, L; Jaksche, H; Hauber, J; Bevec, D;
Indirizzi:
Novartis Res Inst, Dept Immunol, A-1235 Vienna, Austria Novartis Res InstVienna Austria A-1235 Immunol, A-1235 Vienna, Austria Univ Erlangen Nurnberg, Inst Clin & Mol Virol, D-91054 Erlangen, Germany Univ Erlangen Nurnberg Erlangen Germany D-91054 -91054 Erlangen, Germany
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 11, volume: 96, anno: 1999,
pagine: 6229 - 6234
SICI:
0027-8424(19990525)96:11<6229:EFSNTP>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN T-CELLS; IMMUNODEFICIENCY-VIRUS REV; HIV-1 REV; LEPTOMYCIN-B; ACTIVATION DOMAIN; PROTEIN EXPORT; CRM1; INHIBITION; REPLICATION; NUCLEOPORIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Bevec, D Novartis Res Inst, Dept Immunol, Brunner Str 59, A-1235 Vienna, Austria Novartis Res Inst Brunner Str 59 Vienna Austria A-1235 , Austria
Citazione:
C. Elfgang et al., "Evidence for specific nucleocytoplasmic transport pathways used by leucine-rich nuclear export signals", P NAS US, 96(11), 1999, pp. 6229-6234

Abstract

Various proteins with different biological activities have been observed to be translocated from the nucleus to the cytoplasm in an energy- and signal-dependent manner in eukaryotic cells. This nuclear export is directed by nuclear export signals (NESs), typically characterized by hydrophobic, primarily leucine, amino acid residues. Moreover, it has been shown that CRM1/exportin I is an export receptor for leucine-rich NESs. However, additional NES-interacting proteins have been described. In particular, eukaryotic initiation factor 5A (eIF-5A) has been shown to be a critical cellular cofactor for the nuclear export of the HIV type 1 (HIV-I) Rev trans-activator protein. In this study we compared the nuclear export activity of NESs of different origin. Microinjection of export substrates into the nucleus of somatic cells in combination with specific inhibitors indicated that specific nuclear export pathways exist for different NES-containing proteins. In particular, inhibition of eIF-5A blocked the nuclear export of NESs derived from the HIV-1 Rev and human T cell leukemia virus type I Rex transactivators, whereas nucleocytoplasmic translocation of the protein kinase inhibitor-NES was unaffected. In contrast, however, inhibition of CRM/exportin 1 blocked the nuclear export of all NES-containing proteins investigated. Our data confirm that CRM1/exportin 1 is a general export receptor for leucine-rich NESs and suggest that eIF-5A acts either upstream of CRM1/exportin 1 or formsa complex with the NES and CRM1/exportin 1 in the nucleocytoplasmic translocation of the HIV-1 Rev and human T cell leukemia virus type I Rex RNA export factors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 01:47:25