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Titolo:
Pigment recovery from encapsulated-dehydrated Vaccinium pahalae (ohelo) cryopreserved cells
Autore:
Shibli, RA; Smith, MAL; Shatnawi, MA;
Indirizzi:
Jordan Univ Sci & Technol, Ctr Biotechnol, Irbid, Jordan Jordan Univ Sci &Technol Irbid Jordan l, Ctr Biotechnol, Irbid, Jordan Univ Illinois, Dept Nat Resources& Environm Sci, Urbana, IL 61801 USA UnivIllinois Urbana IL USA 61801 ces& Environm Sci, Urbana, IL 61801 USA
Titolo Testata:
PLANT CELL TISSUE AND ORGAN CULTURE
fascicolo: 2, volume: 55, anno: 1998,
pagine: 119 - 123
SICI:
0167-6857(1998)55:2<119:PRFEVP>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOMATIC EMBRYOS; CATHARANTHUS-ROSEUS; LIQUID-NITROGEN; SHOOT TIPS; CULTURES; INVITRO; VITRIFICATION; PRESERVATION; PLANTLETS; GERMPLASM;
Keywords:
anthocyanins; cell culture; cryopreservation; in vitro; pigment; Vaccinium;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Shibli, RA Jordan Univ Sci & Technol, Ctr Biotechnol, Irbid, Jordan JordanUniv Sci & Technol Irbid Jordan echnol, Irbid, Jordan
Citazione:
R.A. Shibli et al., "Pigment recovery from encapsulated-dehydrated Vaccinium pahalae (ohelo) cryopreserved cells", PL CELL TIS, 55(2), 1998, pp. 119-123

Abstract

Pigment producing in vitro cells of Vaccinium pahalae (ohelo) were tested for their ability to survive cryopreservation and retain pigment-productioncapacity after encapsulation-dehydration. Preculture of cells for 6 to 8 days in a medium containing 1.0 M sucrose was essential before dehydration. Reduction of bead water content before quenching in liquid nitrogen was highly correlated (r = 0.94) with increased survival rate in cells after cryopreservation. Dehydration of beads for 4 h was satisfactory for survival of cells. After quenching in liquid nitrogen, colored cells became pale, but pigment content was recovered once cells resumed growth. After three subcultures, cells regained their maximum capacity for pigment accumulation. The percentage of colored-to-total cell volume was not influenced by cryopreservation. Encapsulation-dehydration and cryopreservation did not diminish the capacity of cells to produce anthocyanins and other flavonoids.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 08:49:36