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Titolo:
PH-20 but not acrosin is involved in sperm penetration of the macaque zonapellucida
Autore:
Yudin, AI; Vandevoort, CA; Li, MW; Overstreet, JW;
Indirizzi:
UnivtCalif Davis, Inst Toxicol & Environm Hlth, Div Reprod Biol, Dept Obste Univ Calif Davis Davis CA USA 95616 onm Hlth, Div Reprod Biol, Dept Obste Univ Calif Davis, Calif Reg Primate Res Ctr, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 Primate Res Ctr, Davis, CA 95616 USA
Titolo Testata:
MOLECULAR REPRODUCTION AND DEVELOPMENT
fascicolo: 3, volume: 53, anno: 1999,
pagine: 350 - 362
SICI:
1040-452X(199907)53:3<350:PBNAII>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
GUINEA-PIG SPERM; INNER ACROSOMAL MEMBRANE; HYALURONIDASE ACTIVITY; HUMAN-SPERMATOZOA; PLASMA-MEMBRANE; PROTEIN PH-20; MAMMALIAN FERTILIZATION; HAMSTER SPERMATOZOA; REACTED SPERMATOZOA; CUMULUS PENETRATION;
Keywords:
acrosin; PH-20; CD-46; sperm; zona pellucida;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
70
Recensione:
Indirizzi per estratti:
Indirizzo: Overstreet, JW UnivtCalif Davis, Inst Toxicol & Environm Hlth, Div Reprod Biol, Dept Obste Univ Calif Davis 1 Shields Ave Davis CA USA 95616 ept Obste
Citazione:
A.I. Yudin et al., "PH-20 but not acrosin is involved in sperm penetration of the macaque zonapellucida", MOL REPROD, 53(3), 1999, pp. 350-362

Abstract

In this study, we investigated the functions of PH-20 and acrosin during the interaction of macaque sperm with the zona pellucida. Both of these sperm enzymes have been reported to be present on the inner acrosomal membrane of acrosome reacted sperm, and have been suggested to play a role during secondary sperm-zona binding in other species. Anti-macaque PH-20 IgG, anti-pig acrosin IgG and soybean trypsin inhibitor (SBTI) were used as probes forimmunolocalization of the two proteins at the ultrastructural level, and as reagents for blocking sperm penetration of the macaque zona pellucida in vitro, As a control, we performed similar studies with antibodies to CD-46,which is also located on the inner acrosomal membrane, but has no known function in sperm-zona pellucida interaction, After labeling with anti-acrosin IgG, gold label was not present on the sperm surface before the acrosome reaction, but was detected over the entire head of sperm that were induced to acrosome react with calcium ionophore A23187. In contrast, when sperm were induced to acrosome react by binding to intact zona pellucida, acrosin was present in the acrosomal shroud but not on the inner acrosomal membrane,Similar results were obtained when SBTI was used as a probe for enzyme localization. PH-20 and CD-46 were demonstrated on the inner acrosomal membrane of sperm induced to acrosome react by ionophore treatment and by zona binding. Neither anti-acrosin IgG nor anti-CD-46 IgG affected sperm penetration of the zona at concentrations up to 300 mu g/ml, but zona penetration wasblocked completely when anti-PH-20 IgG (100 mu g/ml) was present during sperm-oocyte interaction, Ultrastructural observations of oocytes incubated with anti-PH-20 IgG showed that acrosomal shrouds were present on the zona surface but no sperm had begun to penetrate into the zona substance. We conclude that anti-PH-20 IgG prevented sperm penetration of the macaque zona pellucida by interference with secondary sperm-zona binding, rather than primary sperm-zona binding or the zona-induced acrosome reaction. Acrosin was not detected on the inner acrosomal membrane of sperm that are induced to acrosome react after zona binding, and acrosin does not appear to be criticalfor sperm penetration of the macaque zona pellucida, Mol. Reprod. Dev, 53:350-362, 1999. (C) 1999 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 12:51:30